Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model
Leong, David, Abraham, Mohan Chothirakottu, Rath, Subha Narayan, Lim, Thiam Chye, Chew, Fook Tim, & Hutmacher, Dietmar (2006) Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model. Differentiation, 74(9/10), pp. 519-529.
The osteogenic potential of human adipose-derived precursor cells seeded on medical-grade polycaprolactone-tricalcium phosphate scaffolds was investigated in this in vivo study. Three study groups were investigated: (1) induced—stimulated with osteogenic factors only after seeding into scaffold; (2) preinduced—induced for 2 weeks before seeding into scaffolds; and (3) uninduced—cells without any introduced induction. For all groups, scaffolds were implanted subcutaneously into the dorsum of athymic rats. The scaffold/cell constructs were harvested at the end of 6 or 12 weeks and analyzed for osteogenesis. Gross morphological examination using scanning electron microscopy indicated good integration of host tissue with scaffold/cell constructs and extensive tissue infiltration into the scaffold interior. Alizarin Red histology and immunostaining showed a heightened level of mineralization and an increase in osteonectin, osteopontin, and collagen type I protein expression in both the induced and preinduced groups compared with the uninduced groups. However, no significant differences were observed in these indicators when compared between the induced and preinduced groups.
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|Item Type:||Journal Article|
|Keywords:||progenitor cells, osteo-differentiation, osteogenesis, animal model, bone tissue engineering|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > OTHER BIOLOGICAL SCIENCES (069900)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering|
|Deposited On:||21 Oct 2010 06:02|
|Last Modified:||29 Feb 2012 13:57|
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