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The application of profluorescent nitroxides to detect reactive oxygen species derived from combustion-generated particulate matter: Cigarette smoke – a case study

Miljevic, Branka, Fairfull-Smith, Kathryn E., Bottle, Steven, & Ristovski, Zoran (2010) The application of profluorescent nitroxides to detect reactive oxygen species derived from combustion-generated particulate matter: Cigarette smoke – a case study. Atmospheric Environment, 44(18), pp. 2224-2230.

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Abstract

Reactive oxygen species (ROS) and related free radicals are considered to be key factors underpinning the various adverse health effects associated with exposure to ambient particulate matter. Therefore, measurement of ROS is a crucial factor for assessing the potential toxicity of particles. In this work, a novel profluorescent nitroxide, BPEAnit, was investigated as a probe for detecting particle-derived ROS. BPEAnit has a very low fluorescence emission due to inherent quenching by the nitroxide group, but upon radical trapping or redox activity, a strong fluorescence is observed. BPEAnit was tested for detection of ROS present in mainstream and sidestream cigarette smoke. In the case of mainstream cigarette smoke, there was a linear increase in fluorescence intensity with an increasing number of cigarette puffs, equivalent to an average of 101 nmol ROS per cigarette based on the number of moles of the probe reacted. Sidestream cigarette smoke sampled from an environmental chamber exposed BPEAnit to much lower concentrations of particles, but still resulted in a clearly detectible increase in fluorescence intensity with sampling time. It was calculated that the amount of ROS was equivalent to 50 ± 2 nmol per mg of particulate matter; however, this value decreased with ageing of the particles in the chamber. Overall, BPEAnit was shown to provide a sensitive response related to the oxidative capacity of the particulate matter. These findings present a good basis for employing the new BPEAnit probe for the investigation of particle-related ROS generated from cigarette smoke as well as from other combustion sources.

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14 citations in Web of Science®

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ID Code: 38668
Item Type: Journal Article
Keywords: Combustion Particles, Cigarette Smoke, Reactive Oxygen Species, Fluroesence, Profluorescent Nitroxides
DOI: 10.1016/j.atmosenv.2010.02.043
ISSN: 13522310
Subjects: Australian and New Zealand Standard Research Classification > EARTH SCIENCES (040000) > ATMOSPHERIC SCIENCES (040100) > Atmospheric Aerosols (040101)
Australian and New Zealand Standard Research Classification > EARTH SCIENCES (040000) > ATMOSPHERIC SCIENCES (040100) > Atmospheric Sciences not elsewhere classified (040199)
Divisions: Past > Schools > Chemistry
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > Physics
Copyright Owner: Copyright 2010 Elsevier
Deposited On: 19 Nov 2010 12:24
Last Modified: 01 Mar 2012 00:25

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