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Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing

Melchels, Ferry P.W., Barradas, Ana M.C. , van Blitterswijk, Clemens A. , de Boer, Jan , Feijen, Jan , & Grijpma, Dirk W. (2010) Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing. Acta Biomaterialia, 6(11), pp. 4208-4217.

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Abstract

The advance of rapid prototyping techniques has significantly improved control over the pore network architecture of tissue engineering scaffolds. In this work we assessed the influence of scaffold pore architecture on cell seeding and static culturing, by comparing a computer‐designed gyroid architecture fabricated by stereolithography to a random‐pore architecture resulting from salt‐leaching. The scaffold types showed comparable porosity and pore size values, but the gyroid type showed a more than tenfold higher permeability due to the absence of size‐limiting pore interconnections. The higher permeability significantly improved the wetting properties of the hydrophobic scaffolds, and increased the settling speed of cells upon static seeding of immortalised mesenchymal stem cells. After dynamic seeding followed by 5 days of static culture, gyroid scaffolds showed large cell populations in the centre of the scaffold, while salt‐leached scaffolds were covered with a cell‐sheet on the outside and no cells were found in the scaffold centre. It was shown that interconnectivity of the pores and permeability of the scaffold prolongs the time of static culture before overgrowth of cells at the scaffold periphery occurs. Furthermore, novel scaffold designs are proposed to further improve the transport of oxygen and nutrients throughout the scaffolds, and to create tissue engineering grafts with designed, pre‐fabricated vasculature.

Impact and interest:

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45 citations in Web of Science®

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ID Code: 38848
Item Type: Journal Article
Keywords: scaffold architecture, computer‐aided design, cell seeding, hypoxia, nutrient transport
DOI: 10.1016/j.actbio.2010.06.012
ISSN: 1742-7061
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Copyright Owner: Copyright 2010 Acta Materialia Inc. Published by Elsevier Ltd.
Deposited On: 24 Nov 2010 14:53
Last Modified: 01 Mar 2012 00:14

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