Molecular characterisation of six badnavirus species associated with leaf streak disease of banana in East Africa
James, Anthony, Geijskes, Robert J., Dale, James L., & Harding, Robert M. (2011) Molecular characterisation of six badnavirus species associated with leaf streak disease of banana in East Africa. Annals of Applied Biology, 158(3), pp. 346-353.
Banana leaf streak disease, caused by several species of Banana streak virus (BSV), is widespread in East Africa. We surveyed for this disease in Uganda and Kenya, and used rolling-circle amplification (RCA) to detect the presence of BSV in banana. Six distinct badnavirus sequences, three from Uganda and three from Kenya, were amplified for which only partial sequences were previously available. The complete genomes were sequenced and characterised. The size and organisation of all six sequences was characteristic of other badnaviruses, including conserved functional domains present in the putative polyprotein encoded by open reading frame (ORF) 3. Based on nucleotide sequence analysis within the reverse transcriptase/ribonuclease H-coding region of open reading frame 3, we propose that these sequences be recognised as six new species and be designated as Banana streak UA virus, Banana streak UI virus, Banana streak UL virus, Banana streak UM virus, Banana streak CA virus and Banana streak IM virus. Using PCR and species-specific primers to test for the presence of integrated sequences, we demonstrated that sequences with high similarity to BSIMV only were present in several banana cultivars which had tested negative for episomal BSV sequences.
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|Item Type:||Journal Article|
|Keywords:||banana streak virus, badnavirus, characterisation, banana, africa|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Virology (060506)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Pathology (060704)
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities
Past > QUT Faculties & Divisions > Faculty of Science and Technology
|Copyright Owner:||Copyright 2011 Wiley-Blackwell|
|Copyright Statement:||The definitive version is available at www3.interscience.wiley.com]|
|Deposited On:||10 Mar 2011 23:04|
|Last Modified:||02 May 2011 00:30|
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