Development of a novel rolling-circle amplification technique to detect Banana streak virus which also discriminates between integrated and episomal virus sequences

James, Anthony, Geijskes, Robert Jason, Dale, James L., & Harding, Robert M. (2010) Development of a novel rolling-circle amplification technique to detect Banana streak virus which also discriminates between integrated and episomal virus sequences. Plant Disease, 95, pp. 57-62.

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Bananas are hosts to a large number of banana streak virus (BSV) species. However, diagnostic methods for BSV are inadequate because of the considerable genetic and serological diversity amongst BSV isolates and the presence of integrated BSV sequences in some banana cultivars which leads to false positives. In this study, a sequence non-specific, rolling-circle amplification (RCA) technique was developed and shown to overcome these limitations for the detection and subsequent characterisation of BSV isolates infecting banana. This technique was shown to discriminate between integrated and episomal BSV DNA, specifically detecting the latter in several banana cultivars known to contain episomal and/or integrated sequences of Banana streak Mysore virus (BSMyV), Banana streak OL virus (BSOLV) and Banana streak GF virus (BSGFV). Using RCA, the presence of BSMyV and BSOLV was confirmed in Australia, while BSOLV, BSGFV, Banana streak Uganda I virus (BSUgIV), Banana streak Uganda L virus (BSUgLV) and Banana streak Uganda M virus (BSUgMV) were detected in Uganda. This is the first confirmed report of episomally-derived BSUglV, BSUgLV and BSUgMV in Uganda. As well as its ability to detect BSV, RCA was shown to detect two other pararetroviruses, Sugarcane bacilliform virus in sugarcane and Cauliflower mosaic virus in turnip.

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29 citations in Scopus
20 citations in Web of Science®
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ID Code: 39057
Item Type: Journal Article
Refereed: Yes
Keywords: banana streak virus, integration, RCA
DOI: 10.1094/PDIS-07-10-0519
ISSN: 0191-2917
Subjects: Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > AGRICULTURAL BIOTECHNOLOGY (100100) > Agricultural Biotechnology Diagnostics (incl. Biosensors) (100101)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2010 Please consult the authors.
Deposited On: 08 Dec 2010 00:58
Last Modified: 20 Dec 2010 05:57

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