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Nuclear localization of NPR1 is required for activation of PR gene expression

Kinkema, Mark, Fan, Weihua, & Dong, Xinnian (2000) Nuclear localization of NPR1 is required for activation of PR gene expression. Plant Cell, 12(12), pp. 2339-2350.

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Abstract

Systemic acquired resistance (SAR) is a broad-spectrum resistance in plants that involves the upregulation of a battery of pathogenesis-related (PR) genes. NPR1 is a key regulator in the signal transduction pathway that leads to SAR. Mutations in NPR1 result in a failure to induce PR genes in systemic tissues and a heightened susceptibility to pathogen infection, whereas overexpression of the NPR1 protein leads to increased induction of the PR genes and enhanced disease resistance. We analyzed the subcellular localization of NPR1 to gain insight into the mechanism by which this protein regulates SAR. An NPR1–green fluorescent protein fusion protein, which functions the same as the endogenous NPR1 protein, was shown to accumulate in the nucleus in response to activators of SAR. To control the nuclear transport of NPR1, we made a fusion of NPR1 with the glucocorticoid receptor hormone binding domain. Using this steroid-inducible system, we clearly demonstrate that nuclear localization of NPR1 is essential for its activity in inducing PR genes.

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243 citations in Web of Science®

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ID Code: 39092
Item Type: Journal Article
Additional Information: Articles free to read on journal website after 12 months
Keywords: NPR1, Disease Resistance, Arabidopsis, Nuclear Localization
DOI: 10.​1105/​tpc.​12.​12.​2339
ISSN: 1040-4651
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Cell and Molecular Biology (060702)
Copyright Owner: Copyright 2010 American Society of Plant Biologists.
Deposited On: 09 Dec 2010 01:15
Last Modified: 29 Jan 2015 04:12

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