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Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR–1 gene

Zhang, Yuelin, Fan, Weihua, Kinkema, Mark, Li, Xin, & Dong, Xinnian (1999) Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR–1 gene. Proceedings of the National Academy of Sciences of the United States of America, 96, pp. 6523-6528.

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    Abstract

    The Arabidopsis thaliana NPR1 has been shown to be a key regulator of gene expression during the onset of a plant disease-resistance response known as systemic acquired resistance. The npr1 mutant plants fail to respond to systemic acquired resistance-inducing signals such as salicylic acid (SA), or express SA-induced pathogenesis-related (PR) genes. Using NPR1 as bait in a yeast two-hybrid screen, we identified a subclass of transcription factors in the basic leucine zipper protein family (AHBP-1b and TGA6) and showed that they interact specifically in yeast and in vitro with NPR1. Point mutations that abolish the NPR1 function in A. thaliana also impair the interactions between NPR1 and the transcription factors in the yeast two-hybrid assay. Furthermore, a gel mobility shift assay showed that the purified transcription factor protein, AHBP-1b, binds specifically to an SA-responsive promoter element of the A. thaliana PR-1 gene. These data suggest that NPR1 may regulate PR-1 gene expression by interacting with a subclass of basic leucine zipper protein transcription factors.

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    309 citations in Scopus
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    291 citations in Web of Science®

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    ID Code: 39094
    Item Type: Journal Article
    Keywords: NPR1, Disease Resistance, Yeast Two Hybrid, Transcription Factor
    ISSN: 1091-6490
    Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > PLANT BIOLOGY (060700) > Plant Cell and Molecular Biology (060702)
    Copyright Owner: Copyright 1999 The National Academy of Sciences
    Deposited On: 09 Dec 2010 08:29
    Last Modified: 22 Feb 2013 12:12

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