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Ovine bone and marrow derived progenitor cells : isolation, characterization, and osteogenic potential

Reichert, Johannes C., Woodruff, Maria A., Friis, Thor, Quent, Verena M.C., Gronthos, Stan, Duda, Georg N., Schutz, Michael A., & Hutmacher, Dietmar W. (2010) Ovine bone and marrow derived progenitor cells : isolation, characterization, and osteogenic potential. Journal of Tissue Engineering and Regenerative Medicine, 4(7), pp. 565-576.

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Abstract

Recently, research has focused on bone marrow derived multipotent mesenchymal precursor cells (MPC) for their potential clinical use in bone engineering. Prior to clinical application, MPC-based treatment concepts need to be evaluated in preclinical, immunocompetent, large animal models. Sheep in particular are considered a valid model for orthopaedic and trauma related research. However, ovine MPC and their osteogenic potential remain poorly characterized. In the present study, ex vivo expanded MPC isolated from ovine bone marrow proliferated at a higher rate than osteoblasts (OB) derived from tibial compact bone as assessed using standard 2D culture. MPC expressed the respective phenotypic profile typical for different mesenchymal cell populations (CD14-/CD31-/CD45- /CD29+/CD44+/CD166+) and showed a multilineage differentiation potential. When compared to OB, MPC had a higher mineralization potential under standard osteogenic culture conditions and expressed typical markers such as osteocalcin, osteonectin and type I collagen at the mRNA and protein level. After 4 weeks in 3D culture, MPC constructs demonstrated higher cell density and mineralization, whilst cell viability on the scaffolds was assessed >90%. Cells displayed a spindle-like morphology and formed an interconnected network. Implanted subcutaneously into NOD/SCID mice on type I collagen coated polycaprolactone-tricalciumphosphate (mPCL-TCP) scaffolds, MPC presented a higher developmental potential than osteoblasts. In summary, this study provides a detailed in vitro characterisation of ovine MPC from a bone engineering perspective and suggests that MPC provide promising means for future bone disease related treatment applications.

Impact and interest:

18 citations in Scopus
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15 citations in Web of Science®

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71 since deposited on 08 Dec 2010
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ID Code: 39108
Item Type: Journal Article
Additional URLs:
Keywords: bone, tissue engineering, PCL, polymer, sheep, scaffold
DOI: 10.1002/term.276
ISSN: 1932-7005
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2010 John Wiley & Sons
Deposited On: 09 Dec 2010 09:16
Last Modified: 01 Mar 2012 00:25

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