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A Survey of Viruses Affecting Dry Bean and Cowpea in Lebanon

Jefferson, Osmat Azzam & Makkouk, Khaled (1985) A Survey of Viruses Affecting Dry Bean and Cowpea in Lebanon. Arab Journal of Plant Protection, 3(2), pp. 76-80.

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Abstract

A survey was conducted to identify viruses affecting dry bean (Phaseolus vulgaris) and cowpea (Vigna unguiculata) in Lebanon. Three hundred and thirty four samples exhibiting virus-like symptoms were collected from 13 different locations during the fall growing season of 1984. Samples were stored at 20 deg C until they were tested by the enzyme-linked immunosorbent assay (ELISA) for the presence of blackeye cowpea mosaic virus (B1CMV), bean yellow mosaic virus (BYMV), bean common mosaic virus (BCMV) and cucumber mosaic virus (CMV). In preliminary tests, the extraction buffer 0.1M phosphate + O.1MEDTA, pH 7.4 was found to be far better than the standard extraction buffer and, accordingly, was used for virus extraction for all field samples. Results obtained indicated that around 50% of the bean samples tested were infected with B1CMV. Incidence of BCMV, BYMV and CMV in the samples tested were 4,4 and 1.7%, respectively. B1CMV was detected in 10 locations, whereas, BYMV, BCMV and CMV were found in 1,4 and 4 locations, respectively. Mixed infections such as BCMV, BICMV, BCMV+CMV, BYMV+CMV and BICMV+BCMV+CMV were detected. In 35% of the samples assayed, the causal virus was not identified

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ID Code: 39783
Item Type: Journal Article
Keywords: Viruses, dry bean, cowpea, Lebanon, Arab, Survey, Jefferson, Osmat, Ossmat, Azzam
ISSN: 0255-983X
Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > HORTICULTURAL PRODUCTION (070600)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 1985 Arab Society for Plant Protection
Deposited On: 10 Mar 2011 08:46
Last Modified: 23 Dec 2012 04:25

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