Detection of dsRNA in Grapevines Showing Symptoms of Rupestris Stem Pitting Disease and the Variabilities Encountered

Azzam, Ossmat I., Gonsalves, Dennis, & Golino, Deborah (1991) Detection of dsRNA in Grapevines Showing Symptoms of Rupestris Stem Pitting Disease and the Variabilities Encountered. Plant Disease : An International Journal of Applied Plant Pathology, 75(9), pp. 960-964.


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Rupestris stem pitting (rSP), a graft-transmissible grapevine disease, can be identified only by its reaction (pitted wood) on inoculated Vitis rupestris ‘St. George.’ DsRNA was extracted from grapevines from California and Canada that indexed positive for rSP on St. George. Two distinct dsRNA species (B and C) (Mr = 5.3 × 106 and 4.4 × 106, respectively) were detected from the stem tissue of rSP-positive samples. Although similar dsRNA species (B and C) were detected in extracts of grapevines from New York, the association of dsRNA B and C with rSP in New York samples was not consistent. Also, eight different dsRNAs, known to be associated with the powdery mildew fungus, Uncinula necator, were detected in leaves of New York samples. In New York, the dsRNAs were not observed in leaves or stem samples collected from June through late August during the 1988 and 1989 growing seasons, suggesting that dsRNA detection in the grape tissue is variable throughout the season. We suggest that dsRNA species B and C are associated with rSP disease. The inconsistent results with New York samples are discussed.

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ID Code: 39795
Item Type: Journal Article
Refereed: Yes
Keywords: Detection of dsRNA, Grapevines, Rupestris stem pitting disease, Variabilities encountered, Jefferson, Osmat, Ossmat, Azzam
DOI: 10.1094/PD-75-0960
ISSN: 0191-2917
Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > HORTICULTURAL PRODUCTION (070600)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Deposited On: 06 Apr 2011 23:50
Last Modified: 21 Dec 2012 11:50

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