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Whitefly Transmission and Efficient ssDNA Accumulation of Bean Golden Mosaic Geminivirus Require Functional Coat Protein

Azzam, O., Frazer, J., De La Rosa, D., Ahlquist, P., & Maxwell, D.P. (1994) Whitefly Transmission and Efficient ssDNA Accumulation of Bean Golden Mosaic Geminivirus Require Functional Coat Protein. Virology, 204(1), pp. 289-296.

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    Abstract

    Bean golden mosaic geminivirus (BGMV) has a bipartite genome composed of two circular ssDNA components (DNA-A and DNA-B) and is transmitted by the whitefly, Bemisia tabaci. DNA-A encodes the viral replication proteins and the coat protein. To determine the role of BGMV coat protein systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the BGMV coat protein gene. All three coat protein mutants produced systemic infections when coinoculated with DNA-B onto Phaseolus vulgaris using electric discharge particle acceleration "particle gun." However, they were not sap transmissible and coat protein was not detected in mutant-infected plants. In addition, none of the mutants were transmitted by whiteflies. With all three mutants, ssDNA accumulation of DNA-A and DNA-B was reduced 25- to 50-fold and 3- to 10-fold, respectively, as compared to that of wild-type DNA. No effect on dsDNA-A accumulation was detected and there was 2- to 5-fold increase in dsDNA-B accumulation. Recombinants between the mutated DNA-A and DNA-B forms were identified when the inoculated coat protein mutant was linearized in the common region.

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    ID Code: 39798
    Item Type: Journal Article
    Keywords: Whitefly transmission, Efficient ssDNA accumulation, Bean golden mosaic geminivirus, Functional coat protein, Jefferson, Osmat, Ossmat, Azzam
    ISSN: 0042-6822
    Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > HORTICULTURAL PRODUCTION (070600)
    Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
    Copyright Owner: Copyright 1994 Academic Press
    Deposited On: 05 Apr 2011 16:24
    Last Modified: 21 Dec 2012 15:02

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