Differentiation of Rice Tungro Bacilliform Virus Strains by Restriction Analysis and DNA Hybridization

Azzam, O., Cabauatan, P.Q., & Arboleda, M. (1998) Differentiation of Rice Tungro Bacilliform Virus Strains by Restriction Analysis and DNA Hybridization. Journal of Virological Methods, 76(1-2), pp. 121-126.

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Abstract

Rice tungro bacilliform virus (RTBV) is one of the two viruses that cause tungro disease. Four RTBV strains maintained in the greenhouse for 4 years, G1, G2, Ic, and L, were differentiated by restriction fragment length polymorphism (RFLP) analysis of the native viral DNA. Although strains G1 and Ic had identical restriction patterns when cleaved with Pst1, BamHI, EcoRI, and EcoRV, they can be differentiated from strains G2 and L by EcoRI and EcoRV digestion. These same endonucleases also differentiate strain G2 from strain L. When total DNA extracts from infected plants were used instead of viral DNA, and digested with EcoRV, identical restriction patterns for each strain (G2 and L) were obtained from roots, leaves, and leaf sheaths of infected plants. The restriction patterns were consistent from plant to plant, in different varieties, and at different times after inoculation. This technique can be used to differentiate RTBV strains and determine the variability of a large number of field samples.

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ID Code: 39800
Item Type: Journal Article
Refereed: Yes
Keywords: Rice tungro, Bacilliform virus strains, DNA hybridization, Restriction analysis, Jefferson, Osmat, Ossmat, Azzam
DOI: 10.1016/S0166-0934(98)00132-3
ISSN: 0166-0934
Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > HORTICULTURAL PRODUCTION (070600)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright © 1998 Elsevier Science B.V. All rights reserved.
Deposited On: 06 Apr 2011 22:41
Last Modified: 27 Dec 2012 00:41

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