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Sourcing fecal pollution from onsite wastewater treatment systems in surface waters using antibiotic resistance analysis

Carroll, Steven P., Hargreaves, Megan, & Goonetilleke, Ashantha (2005) Sourcing fecal pollution from onsite wastewater treatment systems in surface waters using antibiotic resistance analysis. Journal of Applied Microbiology, 99(3), pp. 471-482.

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Abstract

Aims: To identify the sources of fecal contamination in investigated surface waters and determine the significance of onsite wastewater treatment systems (OWTS) as a major contributor to fecal contamination. Methods and Results: Antibiotic Resistance Patterns (ARP) were established for a library of 717 known E. coli source isolates obtained from human, domesticated animals, livestock and wild sources. Eight commonly used antibiotics, including Amoxicillin, Cephalothin, Erythromycin, Gentamicin, Ofloxacin, Chlortetracycline, Tetracycline and Moxalactam, at four different concentrations were used to obtain ARP’s for E. coli isolates. Discriminant Analysis (DA) was used to differentiate between the ARP of sources isolates. The developed ARP library was found to be adequate for discriminating human from non-human isolates, and was used to classify 256 enumerated E. coli isolates collected from monitored surface water locations. Conclusions: The resulting ARP DA indicated that a majority of the fecal contamination in more rural areas was non-human, however the percentage of human isolates increased significantly in urbanised areas using OWTS for wastewater treatment. Significance of Results: This study signifies the feasibility of using antibiotic resistance patterns for source tracking fecal contamination in surface waters, and linking fecal contamination to OWTS. The information will enable regulatory authorities to implement appropriate management practices to reduce the contamination of water resources caused by high densities and failing OWTS.

Impact and interest:

19 citations in Scopus
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16 citations in Web of Science®

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ID Code: 4018
Item Type: Journal Article
Keywords: nsite Systems, E, coli, Antibiotic Resistance, Discriminant Analysis
DOI: 10.1111/j.1365-2672.2005.02644.x
ISSN: 1364-5072
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100) > Quality Assurance Chemometrics Traceability and Metrological Chemistry (030106)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > CIVIL ENGINEERING (090500) > Water Quality Engineering (090508)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > ENVIRONMENTAL ENGINEERING (090700) > Environmental Technologies (090703)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > ENVIRONMENTAL ENGINEERING (090700) > Environmental Engineering Modelling (090702)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Bacteriology (060501)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Copyright Owner: Copyright 2005 Blackwell Publishing
Copyright Statement: The definitive version is available at www.blackwell-synergy.com
Deposited On: 14 Jun 2006
Last Modified: 29 Feb 2012 23:14

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