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Identification of novel markers for uncomplicated lower genital tract infections and upper genital tract pathology due to Chlamydia trachomatis.

Collet, Trudi, McNaughton, Tom, Walsh, Terence P., Debattista, Joseph, & Timms, Peter (2011) Identification of novel markers for uncomplicated lower genital tract infections and upper genital tract pathology due to Chlamydia trachomatis. International Journal of Infectious Diseases, 15(3), pp. 1-10.

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    Abstract

    Background: Untreated Chlamydia trachomatis infections in women can result in disease sequelae such as salpingitis and pelvic inflammatory disease (PID), ultimately culminating in tubal occlusion and infertility. Whilst nucleic acid amplification tests can effectively diagnose uncomplicated lower genital tract (LGT) infections, they are not suitable for diagnosing upper genital tract (UGT) pathological sequelae. As a consequence, this study aimed to identify serological markers that can, with a high degree of sensitivity and specificity, discriminate between LGT infections and UGT pathology. Methods: Plasma was collected from 73 women with a history of LGT infection, UGT pathology due to C. trachomatis or no serological evidence of C. trachomatis infection. Western blotting was used to analyse antibody reactivity against extracted chlamydial proteins. Sensitivity and specificity of differential markers were also calculated. Results: Four antigens (CT157, CT423, CT727 and CT396) were identified and found to be capable of discriminating between the infection and disease sequelae state. Sensitivity and specificity calculations showed that our assay for diagnosing LGT infection had a sensitivity and specificity of 75% and 76% respectively, whilst the assay for identifying UGT pathology demonstrated 80% sensitivity and 86% specificity. Conclusions: The use of these assays could potentially facilitate earlier diagnoses in women suffering UGT pathology due to C. trachomatis.

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    1 citations in Scopus
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    ID Code: 40200
    Item Type: Journal Article
    Additional URLs:
    Keywords: Chlamydia trachomatis, pelvic inflammatory disease, serology, sensitivity, specificity, antigen
    DOI: 10.1016/j.ijid.2010.12.005
    ISSN: 1878-3511
    Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Infectious Agents (060502)
    Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
    Past > Schools > Pharmacy
    Copyright Owner: Copyright 2011 International Society for Infectious Diseases.
    Deposited On: 21 Feb 2011 09:01
    Last Modified: 11 Aug 2011 02:05

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