Identification of novel markers for uncomplicated lower genital tract infections and upper genital tract pathology due to Chlamydia trachomatis.

Collet, Trudi, McNaughton, Tom, Walsh, Terence Patrick, Debattista, Joseph, & Timms, Peter (2011) Identification of novel markers for uncomplicated lower genital tract infections and upper genital tract pathology due to Chlamydia trachomatis. International Journal of Infectious Diseases, 15(3), pp. 1-10.

[img] Figure 1 (MS Word 5MB)
Supplemental Material.
[img] Figure 2 (MS Word 803kB)
Supplemental Material.
[img] Figure 3 (MS Word 145kB)
Supplemental Material.
[img] Table 1 (MS Word 47kB)
Supplemental Material.
[img] Table 2 (MS Word 79kB)
Supplemental Material.
[img] Tale 3 (MS Word 77kB)
Supplemental Material.
[img] Table 4 (MS Word 72kB)
Supplemental Material.
[img] Table 5 (MS Word 81kB)
Supplemental Material.
[img] Table 6 (MS Word 77kB)
Supplemental Material.
PDF (128kB)

View at publisher


Background: Untreated Chlamydia trachomatis infections in women can result in disease sequelae such as salpingitis and pelvic inflammatory disease (PID), ultimately culminating in tubal occlusion and infertility. Whilst nucleic acid amplification tests can effectively diagnose uncomplicated lower genital tract (LGT) infections, they are not suitable for diagnosing upper genital tract (UGT) pathological sequelae. As a consequence, this study aimed to identify serological markers that can, with a high degree of sensitivity and specificity, discriminate between LGT infections and UGT pathology. Methods: Plasma was collected from 73 women with a history of LGT infection, UGT pathology due to C. trachomatis or no serological evidence of C. trachomatis infection. Western blotting was used to analyse antibody reactivity against extracted chlamydial proteins. Sensitivity and specificity of differential markers were also calculated. Results: Four antigens (CT157, CT423, CT727 and CT396) were identified and found to be capable of discriminating between the infection and disease sequelae state. Sensitivity and specificity calculations showed that our assay for diagnosing LGT infection had a sensitivity and specificity of 75% and 76% respectively, whilst the assay for identifying UGT pathology demonstrated 80% sensitivity and 86% specificity. Conclusions: The use of these assays could potentially facilitate earlier diagnoses in women suffering UGT pathology due to C. trachomatis.

Impact and interest:

3 citations in Scopus
2 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

322 since deposited on 20 Feb 2011
5 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 40200
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Chlamydia trachomatis, pelvic inflammatory disease, serology, sensitivity, specificity, antigen
DOI: 10.1016/j.ijid.2010.12.005
ISSN: 1878-3511
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Infectious Agents (060502)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Past > Schools > Pharmacy
Copyright Owner: Copyright 2011 International Society for Infectious Diseases.
Deposited On: 20 Feb 2011 23:01
Last Modified: 25 Mar 2015 03:40

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page