A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP
Pagan, Julia K. , Arnold, Jeremy , Hanchard, Kim J. , Kumar, Raman , Bruno, Tiziana , Jones, Mathew J. K. , Richard, Derek J., Forrest, Alistair , Spurdle, Amanda B., Verdin, Eric , Crossley, Merlin , Fanciulli, Maurizio , Chenevix-Trench, Georgia, Young, David B. , & Khanna, Kum Kum (2007) A novel corepressor, BCoR-L1, represses transcription through an interaction with CtBP. Journal of Biological Chemistry, 282(20), pp. 15248-15257.
Corepressors play a crucial role in negative gene regulation and are defective in several diseases. BCoR is a corepressor for the BCL6 repressor protein. Here we describe and functionally characterize BCoR-L1, a homolog of BCoR. When tethered to a heterologous promoter, BCoR-L1 is capable of strong repression. Like other corepressors, BCoR-L1 associates with histone deacetylase (HDAC) activity. Specifically, BCoR-L1 coprecipitates with the Class II HDACs, HDAC4, HDAC5, and HDAC7, suggesting that they are involved in its role as a transcriptional repressor. BCoR-L1 also interacts with the CtBP corepressor through a CtBP-interacting motif in its amino terminus. Abrogation of the CtBP binding site within BCoR-L1 partially relieves BCoR-L1-mediated transcriptional repression. Furthermore, BCoR-L1 is located on the E-cadherin promoter, a known CtBP-regulated promoter, and represses the E-cadherin promoter activity in a reporter assay. The inhibition of BCoR-L1 expression by RNA-mediated interference results in derepression of E-cadherin in cells that do not normally express E-cadherin, indicating that BCoR-L1 contributes to the repression of an authentic endogenous CtBP target.
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|Item Type:||Journal Article|
|Keywords:||negative gene regulation, BCL6 repressor protein, BCoR-L1|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2007 American Society for Biochemistry and Molecular Biology|
|Deposited On:||09 Mar 2011 14:09|
|Last Modified:||01 Mar 2012 00:31|
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