Physical and functional interaction of the archaeal single-stranded DNA-binding protein SSB with RNA polymerase

Richard, D.J. (2004) Physical and functional interaction of the archaeal single-stranded DNA-binding protein SSB with RNA polymerase. Nucleic Acids Research, 32(3), pp. 1065-1074.

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Archaeal transcription utilizes a complex multisubunit RNA polymerase and the basal transcription factors TBP and TF(II)B, closely resembling its eukaryal counterpart. We have uncovered a tight physical and functional interaction between RNA polymerase and the single-stranded DNA-binding protein SSB in Sulfolobus solfataricus. SSB stimulates transcription from promoters in vitro under TBP-limiting conditions and supports transcription in the absence of TBP. SSB also rescues transcription from repression by reconstituted chromatin. We demonstrate the potential for promoter melting by SSB, suggesting a plausible basis for the stimulation of transcription. This stimulation requires both the single-stranded DNA-binding domain and the acidic C-terminal tail of the SSB. The tail forms a stable interaction with RNA polymerase. These data reveal an unexpected role for single-stranded DNA-binding proteins in transcription in archaea.

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40 citations in Scopus
39 citations in Web of Science®
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ID Code: 40621
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website
Keywords: Archaeal transcription, RNA polymerase, Sulfolobus solfataricus
DOI: 10.1093/nar/gkh259
ISSN: 1362-4962
Copyright Owner: Copyright 2004 Oxford University Press 2004. All rights reserved
Deposited On: 09 Mar 2011 08:00
Last Modified: 29 Jan 2015 01:40

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