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The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of ureaplasma parvum

Knox, Christine L., Dando, Samantha J., Nitsos, Ilias , Kallapur, Suhas G. , Jobe, Alan H. , Payton, Diane , Moss, Timothy J.M. , & Newnham, John P. (2010) The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of ureaplasma parvum. Biology of Reproduction, 83(3), pp. 415-426.

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    Abstract

    Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid in asymptomatic pregnancies and placental infections. Ureaplasma parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects on the fetus and chorioamnion of chronic ureaplasma infection in amniotic fluid are dependent on the serovar, dose, and variation of the ureaplasma multiple banded antigen (MBA) and mba gene. We injected high- or low dose U. parvum serovar 3, serovar 6, or vehicle intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and Western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005),and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (five or fewer) within amniotic fluid were associated with severe inflammation, whereas the chorioamnion from animals with nine or more mba variants showed little or no inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes.

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    16 citations in Scopus
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    14 citations in Web of Science®

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    ID Code: 41040
    Item Type: Journal Article
    Additional URLs:
    Keywords: chorioamnionitis, immunology, multiple-banded antigen gene, pregnancy, Ureaplasma species
    DOI: 10.1095/biolreprod.109.083121
    ISSN: 0006-3363
    Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300)
    Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > MEDICAL MICROBIOLOGY (110800)
    Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > PAEDIATRICS AND REPRODUCTIVE MEDICINE (111400)
    Divisions: Past > Schools > Cell & Molecular Biosciences
    Past > QUT Faculties & Divisions > Faculty of Science and Technology
    Current > Institutes > Institute of Health and Biomedical Innovation
    Copyright Owner: Copyright 2010 Society for the Study of Reproduction, Inc
    Deposited On: 30 Mar 2011 08:43
    Last Modified: 06 Mar 2012 01:13

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