Agroinoculation Allows the Screening of Rice for Resistance to Rice Tungro Bacilliform Virus

Cruz, F. C. StA., Boulton, M. I., Hull, R., & Azzam, O. (1999) Agroinoculation Allows the Screening of Rice for Resistance to Rice Tungro Bacilliform Virus. Journal of Phytopathology, 147(11-12), pp. 653-659.

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A system for agroinoculating rice tungro bacilliform virus (RTBV), one of the two viruses of the rice tungro disease complex, has been optimised. A nontumour-inducing strain of Agrobacterium (pGV3850) was used in order to conform with biosafety regulations. Fourteen-day-old seedlings survived the mechanical damage of the technique and were still young enough to support virus replication. The level of the bacterial inoculum was important to obtain maximum infection, with a high inoculum level (0.5 × 1012 cells/ml) resulting in up to 100% infection of a susceptible variety that was comparable with infection by insect transmission. Agroinoculation with RTBV was successful for all three rice cultivarss tested; TN1 (tungro susceptible), Balimau Putih (tungro tolerant), and IR26 (RTSV and vector resistant). Agroinoculation enables resistance to RTBV to be distinguished from resistance to the leafhopper vector of the virus, and should prove useful in screening rice germplasm, breeding materials, and transgenic rice lines.

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7 citations in Web of Science®

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ID Code: 41106
Item Type: Journal Article
Refereed: Yes
Keywords: agroinoculation, rice tungro bacilliform virus, screening for resistance, Jefferson, Osmat, Ossmat, Azzam
DOI: 10.1046/j.1439-0434.1999.00452.x
ISSN: 0931-1785
Subjects: Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > HORTICULTURAL PRODUCTION (070600)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 1999 Blackwell Wissenschafts-Verlag, Berlin
Deposited On: 04 Apr 2011 22:52
Last Modified: 27 Dec 2012 00:41

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