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Biaxial cell stimulation : a mechanical validation

Bieler, F.H., Ott, C.E., Thompson, M.S., Seidel, R., Ahrens, S., Epari, Devakara R., Wilkening, U., Schaser, K.D., Mundlos, S., & Duda, G.N. (2009) Biaxial cell stimulation : a mechanical validation. Journal of Biomechanics, 42(11), pp. 1692-1696.

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Abstract

To analyse mechanotransduction resulting from tensile loading under defined conditions, various devices for in vitro cell stimulation have been developed. This work aimed to determine the strain distribution on the membrane of a commercially available device and its consistency with rising cycle numbers, as well as the amount of strain transferred to adherent cells. The strains and their behaviour within the stimulation device were determined using digital image correlation (DIC). The strain transferred to cells was measured on eGFP-transfected bone marrow-derived cells imaged with a fluorescence microscope. The analysis was performed by determining the coordinates of prominent positions on the cells, calculating vectors between the coordinates and their length changes with increasing applied tensile strain. The stimulation device was found to apply homogeneous (mean of standard deviations approx. 2% of mean strain) and reproducible strains in the central well area. However, on average, only half of the applied strain was transferred to the bone marrow-derived cells. Furthermore, the strain measured within the device increased significantly with an increasing number of cycles while the membrane's Young's modulus decreased, indicating permanent changes in the material during extended use. Thus, strain magnitudes do not match the system readout and results require careful interpretation, especially at high cycle numbers.

Impact and interest:

20 citations in Scopus
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18 citations in Web of Science®

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ID Code: 41297
Item Type: Journal Article
DOI: 10.1016/j.jbiomech.2009.04.013
ISSN: 00219290
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
Deposited On: 15 Apr 2011 14:59
Last Modified: 29 Feb 2012 23:56

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