Immunohistochemical analysis of structural changes in collagen for the assessment of osteoarthritis
Tian, Y., Peng, Z., Corton, D., Xiao, Y., & Ketheesam, N. (2011) Immunohistochemical analysis of structural changes in collagen for the assessment of osteoarthritis. Institution of Mechanical Engineers. Proceedings. Part H: Journal of Engineering in Medicine, 225(7), pp. 680-687.
Collagen fibrillation within articular cartilage (AC) plays a key role in joint osteoarthritis (OA) progression and, therefore, studying collagen synthesis changes could be an indicator for use in the assessment of OA. Various staining techniques have been developed and used to determine the collagen network transformation under microscopy. However, because collagen and proteoglycan coexist and have the same index of refraction, conventional methods for specific visualization of collagen tissue is difficult. This study aimed to develop an advanced staining technique to distinguish collagen from proteoglycan and to determine its evolution in relation to OA progression using optical and laser scanning confocal microscopy (LSCM). A number of AC samples were obtained from sheep joints, including both healthy and abnormal joints with OA grades 1 to 3. The samples were stained using two different trichrome methods and immunohistochemistry (IHC) to stain both colourimetrically and with fluorescence. Using optical microscopy and LSCM, the present authors demonstrated that the IHC technique stains collagens only, allowing the collagen network to be separated and directly investigated. Fluorescently-stained IHC samples were also subjected to LSCM to obtain three-dimensional images of the collagen fibres. Changes in the collagen fibres were then correlated with the grade of OA in tissue. This study is the first to successfully utilize the IHC staining technique in conjunction with laser scanning confocal microscopy. This is a valuable tool for assessing changes to articular cartilage in OA.
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|Item Type:||Journal Article|
|Keywords:||collagen structure, trichrome staining, immunohistochemical staining technique, optical and laser scanning confocal microscopy, osteoarthritis, articular cartilage|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering|
Current > Institutes > Institute of Health and Biomedical Innovation
Past > Schools > School of Engineering Systems
|Copyright Owner:||Copyright 2011 Sage Publications Ltd.|
|Deposited On:||11 Aug 2011 11:32|
|Last Modified:||09 Nov 2011 12:56|
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