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Adhesion of perichondrial cells to a polylactic acid scaffold

Giurea, A., Klein, Travis J., Chen, A. C., Goomer, R. S., Coutts, R. D., Akeson, W. H., Amiel, D., & Sah, R. L. (2003) Adhesion of perichondrial cells to a polylactic acid scaffold. Journal of Orthopaedic Research : a Journal for Musculoskeletal Investigation, 21(4), pp. 584-589.

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Abstract

The number of chondrogenic cells available locally is an, important factor in the repair process for cartilage defects. Previous studies demonstrated that the number of transplanted rabbit perichondrial cells (PC) remaining in a cartilage defect in vivo, after being carried into the site in a polylactic acid (PLA) scaffold, declined markedly within two days. This study examined the ability of in vitro culture of PC/PLA constructs to enhance subsequent biomechanical stability of the cells and the matrix content in an in vitro screening assay. PC/PLA constructs were analyzed after 1 h, 1 and 2 weeks of culture. The biomechanical adherence of PC to the PLA scaffold was tested by subjecting the PC/PLA constructs to a range of flow velocities (0.25-25 mm/s), spanning the range estimated to occur under conditions of construct insertion in vivo. The adhesion of PC to the PLA carrier was increased significantly by 1 and 2 weeks of incubation, with 25 mm/s flow causing a 57% detachment of cells after 1 h of seeding, but only 7% and 16% after I and 2 weeks of culture, respectively (p < 0.001). This adherence was associated with marked deposition of glycosaminoglycan and collagen. These findings suggest that pre-incubation of PC-laden PLA scaffolds markedly enhances the stability of the indwelling cells. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.

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17 citations in Web of Science®

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ID Code: 41594
Item Type: Journal Article
Keywords: ARTICULAR-CARTILAGE DEFECTS, FULL-THICKNESS DEFECTS, CULTURED CHONDROCYTES, TRANSPLANTATION, REGENERATION; ASSAY
DOI: 10.1016/S0736-0266(02)00263-2
ISSN: 0736-0266
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Biochemistry and Cell Biology not elsewhere classified (060199)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2003 John Wiley and Sons
Deposited On: 26 Aug 2011 10:40
Last Modified: 26 Aug 2011 10:40

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