Zonal chondrocyte subpopulations reacquire zone-specific characteristics during in Vitro redifferentiation
Schuurman, Wouter, Gawlitta, Debby, Klein, Travis J., ten Hoope, Werner, van Rijen, Mattie H. P., Dhert, Wouter J. A., van Weeren, P. Rene, & Malda, Jos (2009) Zonal chondrocyte subpopulations reacquire zone-specific characteristics during in Vitro redifferentiation. American Journal of Sports Medicine, 37(Suppl.), 97S-104S.
Background: If chondrocytes from the superficial, middle, and deep zones of articular cartilage could maintain or regain their characteristic properties during in vitro culture, it would be feasible to create constructs comprising these distinctive zones. ----- -----
Hypothesis: Zone-specific characteristics of zonal cell populations will disappear during 2-dimensional expansion but will reappear after 3-dimensional redifferentiation, independent of the culture technique used (alginate beads versus pellet culture).----- -----
Study Design: Controlled laboratory study.----- -----
Methods: Equine articular chondrocytes from the 3 zones were expanded in monolayer culture (8 donors) and subsequently redifferentiated in pellet and alginate bead cultures for up to 4 weeks. Glycosaminoglycans and DNA were quantified, along with immunohistochemical assessment of the expression of various zonal markers, including cartilage oligomeric protein (marking cells from the deeper zones) and clusterin (specifically expressed by superficial chondrocytes).----- -----
Results: Cell yield varied between zones, but proliferation rates did not show significant differences. Expression of all evaluated zonal markers was lost during expansion. Compared to the alginate bead cultures, pellet cultures showed a higher amount of glycosaminoglycans produced per DNA after redifferentiation. In contrast to cells in pellet cultures, cells in alginate beads regained zonal differences, as evidenced by zone-specific reappearance of cartilage oligomeric protein and clusterin, as well as significantly higher glycosaminoglycans production by cells from the deep zone compared to the superficial zone.----- -----
Conclusion: Chondrocytes isolated from the 3 zones of equine cartilage can restore their zone-specific matrix expression when cultured in alginate after in vitro expansion.
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|Item Type:||Journal Article|
|Additional Information:||Schuurman, Wouter Gawlitta, Debby Klein, Travis J. ten Hoope, Werner van Rijen, Mattie H. P. Dhert, Wouter J. A. van Weeren, P. Rene Malda, Jos
|Keywords:||tissue-engineered cartilage; human articular chondrocytes; oligomeric matrix protein; gene-expression; superficial zone; pellet culture; growth; defects; knee; implantation|
|Subjects:||Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomechanical Engineering (090302)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomedical Engineering not elsewhere classified (090399)
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
|Deposited On:||08 May 2011 23:44|
|Last Modified:||29 Feb 2012 13:59|
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