Study of the interaction between 10-hydroxycamptothecine and DNA with the use of ethidium bromide dye as a fluorescence probe

Ni, Y., Wang, Y., & Kokot, S. (2011) Study of the interaction between 10-hydroxycamptothecine and DNA with the use of ethidium bromide dye as a fluorescence probe. Sensors and Actuators, B: Chemical, 156(1), pp. 290-298.

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Abstract

The interaction of 10-hydroxycamptothecine (HCPT) with DNA under pseudo-physiological conditions (Tris-HCl buffer of pH 7.4), using ethidium bromide (EB) dye as a probe, was investigated with the use of spectrofluorimetry, UV-vis spectrometry and viscosity measurement. The binding constant and binding number for HCPT with DNA were evaluated as (7.1 ± 0.5) × 104 M-1 and 1.1, respectively, by multivariate curve resolution-alternating least squares (MCR-ALS). Moreover, parallel factor analysis (PARAFAC) was applied to resolve the three-way fluorescence data obtained from the interaction system, and the concentration information for the three components of the system at equilibrium was simultaneously obtained. It was found that there was a cooperative interaction between the HCPT-DNA complex and EB, which produced a ternary complex of HCPT-DNA-EB. © 2011 Elsevier B.V.

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ID Code: 42154
Item Type: Journal Article
Refereed: Yes
Keywords: 10-Hydroxycamptothecine, DNA, Ethidium bromide, MCR-ALS, PARAFAC, Spectroscopy
DOI: 10.1016/j.snb.2011.04.035
ISSN: 09254005
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Elsevier
Deposited On: 24 Jun 2011 02:45
Last Modified: 24 Jun 2011 02:45

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