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Long term survival following the detection of circulating tumour cells in head and neck squamous cell carcinoma

Winter, Sutart , Stephenson, Sally-Anne, Subramaniam, Selva , Paleri, Vinidh , Ha, Kien , Marnane, Conor , Krishnan, Suren , & Rees, Guy (2009) Long term survival following the detection of circulating tumour cells in head and neck squamous cell carcinoma. BMC Cancer, 9(424), pp. 1-15.

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Abstract

Background

Techniques for detecting circulating tumor cells in the peripheral blood of patients with head and neck cancers may identify individuals likely to benefit from early systemic treatment. Methods

Reconstruction experiments were used to optimise immunomagnetic enrichment and RT-PCR detection of circulating tumor cells using four markers (ELF3, CK19, EGFR and EphB4). This method was then tested in a pilot study using samples from 16 patients with advanced head and neck carcinomas. Results

Seven patients were positive for circulating tumour cells both prior to and after surgery, 4 patients were positive prior to but not after surgery, 3 patients were positive after but not prior to surgery and 2 patients were negative. Two patients tested positive for circulating cells but there was no other evidence of tumor spread. Given this patient cohort had mostly advanced disease, as expected the detection of circulating tumour cells was not associated with significant differences in overall or disease free survival. Conclusion

For the first time, we show that almost all patients with advanced head and neck cancers have circulating cells at the time of surgery. The clinical application of techniques for detection of spreading disease, such as the immunomagnetic enrichment RT-PCR analysis used in this study, should be explored further.

Impact and interest:

4 citations in Scopus
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4 citations in Web of Science®

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ID Code: 43265
Item Type: Journal Article
Keywords: Immunobead RT-PCR, Marker, Head and neck cancer, Circulating, Tumour Cells
DOI: 10.1186/1471-2407-9-424
ISSN: 1471-2407
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Gene Expression (incl. Microarray and other genome-wide approaches) (060405)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Diagnosis (111202)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Solid Tumours (111209)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 13 Jul 2011 23:11
Last Modified: 03 Dec 2012 12:08

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