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A collagen network phase improves cell seeding of open-pore structure scaffolds under perfusion

Papadimitropoulos, Adam, Riboldi, Stephanie, Tonnarelli, Beatrice, Woodruff, Maria A., Hutmacher, Dietmar W., & Martin, Ivan (2013) A collagen network phase improves cell seeding of open-pore structure scaffolds under perfusion. Journal of Tissue Engineering and Regenerative Medicine, 7(3), pp. 183-191.

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Abstract

Scaffolds with open-pore morphologies offer several advantages in cell-based tissue engineering, but their use is limited by a low cell seeding efficiency. We hypothesized that inclusion of a collagen network as filling material within the open-pore architecture of polycaprolactone-tricalcium phosphate (PCL-TCP) scaffolds increases human bone marrow stromal cells (hBMSC) seeding efficiency under perfusion and in vivo osteogenic capacity of the resulting constructs. PCL-TCP scaffolds, rapid prototyped with a honeycomb-like architecture, were filled with a collagen gel and subsequently lyophilized, with or without final crosslinking. Collagen-free scaffolds were used as controls. The seeding efficiency was assessed after overnight perfusion of expanded hBMSC directly through the scaffold pores using a bioreactor system. By seeding and culturing freshly harvested hBMSC under perfusion for 3 weeks, the osteogenic capacity of generated constructs was tested by ectopic implantation in nude mice. The presence of the collagen network, independently of the crosslinking process, significantly increased the cell seeding efficiency (2.5-fold), and reduced the loss of clonogenic cells in the supernatant. Although no implant generated frank bone tissue, possibly due to the mineral distribution within the scaffold polymer phase, the presence of a non crosslinked collagen phase led to in vivo formation of scattered structures of dense osteoids. Our findings verify that the inclusion of a collagen network within open morphology porous scaffolds improves cell retention under perfusion seeding. In the context of cell-based therapies, collagen-filled porous scaffolds are expected to yield superior cell utilization, and could be combined with perfusion-based bioreactor devices to streamline graft manufacture.

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3 citations in Web of Science®

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ID Code: 43454
Item Type: Journal Article
Keywords: bone tissue engineering , mesenchymal stem cells , bioreactor, biotechnology, cell culture
DOI: 10.1002/term.506
ISSN: 1932-7005
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2011 John Wiley & Sons, Ltd
Deposited On: 22 Jul 2011 09:10
Last Modified: 06 Nov 2013 15:41

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