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Glycosaminoglycan and growth factor mediated murine calvarial cell proliferation

Manton, Kerry, Haupt, Larisa M., Vengadasalam, Kumeri, Nurcombe, Victor, & Cool, Simon M. (2007) Glycosaminoglycan and growth factor mediated murine calvarial cell proliferation. Journal of Molecular Histology, 38(5), pp. 415-424.

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Abstract

Understanding the complex mechanisms underlying bone remodeling is crucial to the development of novel therapeutics. Glycosaminoglycans (GAGs) localised to the extracellular matrix (ECM) of bone are thought to play a key role in mediating aspects of bone development. The influence of isolated GAGs was studied by utilising in vitro murine calvarial monolayer and organ culture model systems. Addition of GAG preparations extracted from the cell surface of human osteoblasts at high concentrations (5 microg/ml) resulted in decreased proliferation of cells and decreased suture width and number of bone lining cells in calvarial sections. When we investigated potential interactions between the growth factors fibroblast growth factor-2 (FGF2), bone morphogenic protein-2 (BMP2) and transforming growth factor-beta1 (TGFbeta1) and the isolated cell surface GAGs, differences between the two model systems emerged. The cell culture system demonstrated a potentiating role for the isolated GAGs in the inhibition of FGF2 and TGFbeta1 actions. In contrast, the organ culture system demonstrated an enhanced stimulation of TFGbeta1 effects. These results emphasise the role of the ECM in mediating the interactions between GAGs and growth factors during bone development and suggest the GAG preparations contain potent inhibitory or stimulatory components able to mediate growth factor activity.

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12 citations in Scopus
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10 citations in Web of Science®

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ID Code: 44255
Item Type: Journal Article
Additional Information: Manton, Kerry J Haupt, Larisa M Vengadasalam, Kumeri Nurcombe, Victor Cool, Simon M eng Research Support, Non-U.S. Gov't Netherlands 2007/07/27 09:00 J Mol Histol. 2007 Oct;38(5):415-24. Epub 2007 Jul 25.
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Keywords: Osteoblast, Calvaria, Organ Culture, FGF2, BMP2, animals, Bone Morphogenetic Protein 2, Bone Morphogenetic Proteins/pharmacology, Cell Differentiation/drug effects, Cell Proliferation/*drug effects, cells, cultured, Dose-Response Relationship, drug, Fibroblast Growth Factor 2/pharmacology, Glycosaminoglycans/*pharmacology, humans, Intercellular Signaling Peptides and Proteins/*, mice, Inbred BALB C, Inbred C57BL, organ culture techniques, Simvastatin/pharmacology, Skull/cytology, Transforming Growth Factor beta/pharmacology, Transforming Growth Factor beta1/pharmacology
DOI: 10.1007/s10735-007-9121-6
ISSN: 1567-2387
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cell Development Proliferation and Death (060103)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CARDIOVASCULAR MEDICINE AND HAEMATOLOGY (110200)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 25 Aug 2011 08:09
Last Modified: 02 Jul 2014 15:22

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