Evidence for u.v. induction of CDKN2 mutations in melanoma cell lines

Pollock, P.M., Yu, F., Qiu, L., Parsons, P.G., & Hayward, N.K. (1995) Evidence for u.v. induction of CDKN2 mutations in melanoma cell lines. Oncogene, 11(4), pp. 663-668.

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The CDKN2 gene, encoding the cyclin dependent kinase inhibitor p16, is a tumour suppressor gene involved in melanoma and maps to chromosome band 9p22. Mutations or interstitial deletions of this gene have been found both in the germline of familial melanoma cases and somatically in melanoma cell lines. Previous mutation analyses of melanoma cell lines have indicated a high frequency of C:G to T:A transitions, with all of these mutations occurring at dipyrimidine sites. Including three melanoma cell lines carrying tandem CC to TT mutations, the spectrum of mutations so far reported indicates a possible role for u.v. radiation in the mutagenesis of this gene in some tumours. To further examine this hypothesis we have characterised mutations of the CDKN2 gene in 30 melanoma cell lines. Nineteen lines carried complete or partial homozygous deletions of the gene. Of the remaining cell lines, eight were shown by direct sequencing of PCR products from exon 1 and exon 2 to carry a total of nine different mutations of CDKN2. Two cell lines carried tandem CC to TT mutations and a high rate of C:G to T:A transitions was observed. This study provides further evidence for the role of u.v. light in the genesis of melanoma, with one target being the CDKN2 tumour suppressor gene.

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ID Code: 45806
Item Type: Journal Article
Refereed: Yes
Keywords: Base Sequence, Carrier Proteins/biosynthesis/ genetics, Cell Line, Cyclin-Dependent Kinase Inhibitor p16, DNA Primers, Exons, Gene Deletion, Genes, Tumor Suppressor/ radiation effects, Homozygote, Humans, Melanoma/ genetics, Molecular Sequence Data, Mutagenesis, Mutation, Polymerase Chain Reaction, Tumor Cells, Cultured, Ultraviolet Rays
ISSN: 0950-9232
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Divisions: Past > Schools > Cell & Molecular Biosciences
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 09 Sep 2011 02:55
Last Modified: 09 Sep 2011 02:55

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