QUT ePrints

Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines

Pollock, Pamela M. & Hayward, Nicholas K. (2002) Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines. Melanoma Research, 12(2), pp. 183-186.

[img] Published Version (PDF 850kB)
Administrators only | Request a copy from author

    View at publisher

    Abstract

    Mutations in exon 3 of the CTNNB1 gene encoding beta-catenin have been reported in colorectal cancer cell lines and tumours. Although one study reported mutations or deletions affecting beta-catenin in 20% of melanoma cell lines, subsequent reports detected a much lower frequency of aberrations in uncultured melanomas. To determine whether this difference in mutation frequency reflected an in vitro culturing artefact, exon 3 of CTNNB1 was screened in a panel of 62 melanoma cell lines. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect intragenic deletions affecting exon 3. One out of 62 (1.6%) cell lines was found to carry a mutation, indicating that aberration of the Wnt-1/wingless pathway through activation of beta-catenin is a rare event, even in melanoma cell lines.

    Impact and interest:

    25 citations in Scopus
    Search Google Scholar™
    23 citations in Web of Science®

    Citation countsare sourced monthly from Scopus and Web of Science® citation databases.

    These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

    Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

    ID Code: 45819
    Item Type: Journal Article
    Additional Information: Pollock, P M Hayward, N England Melanoma research Melanoma Res. 2002 Apr;12(2):183-6.
    Additional URLs:
    Keywords: Cytoskeletal Proteins/ genetics, DNA Mutational Analysis, DNA, Neoplasm/genetics, Exons/genetics, Humans, Melanoma/ genetics/metabolism/pathology, Mutation/ genetics, Polymorphism, Single-Stranded Conformational, Reverse Transcriptase Polymerase Chain Reaction, Skin Neoplasms/ genetics/metabolism/pathology, Trans-Activators, Tumor Cells, Cultured, beta Catenin
    ISSN: 0960-8931
    Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)
    Divisions: Past > Schools > Cell & Molecular Biosciences
    Past > QUT Faculties & Divisions > Faculty of Science and Technology
    Current > Institutes > Institute of Health and Biomedical Innovation
    Copyright Owner: Copyright 2002 Lippincott, Williams & Wilkins
    Deposited On: 14 Sep 2011 09:10
    Last Modified: 18 Sep 2011 10:22

    Export: EndNote | Dublin Core | BibTeX

    Repository Staff Only: item control page