Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines
Pollock, Pamela M. & Hayward, Nicholas K. (2002) Mutations in exon 3 of the beta-catenin gene are rare in melanoma cell lines. Melanoma Research, 12(2), pp. 183-186.
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Mutations in exon 3 of the CTNNB1 gene encoding beta-catenin have been reported in colorectal cancer cell lines and tumours. Although one study reported mutations or deletions affecting beta-catenin in 20% of melanoma cell lines, subsequent reports detected a much lower frequency of aberrations in uncultured melanomas. To determine whether this difference in mutation frequency reflected an in vitro culturing artefact, exon 3 of CTNNB1 was screened in a panel of 62 melanoma cell lines. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect intragenic deletions affecting exon 3. One out of 62 (1.6%) cell lines was found to carry a mutation, indicating that aberration of the Wnt-1/wingless pathway through activation of beta-catenin is a rare event, even in melanoma cell lines.
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|Item Type:||Journal Article|
|Additional Information:||Pollock, P M Hayward, N England Melanoma research Melanoma Res. 2002 Apr;12(2):183-6.|
|Keywords:||Cytoskeletal Proteins/ genetics, DNA Mutational Analysis, DNA, Neoplasm/genetics, Exons/genetics, Humans, Melanoma/ genetics/metabolism/pathology, Mutation/ genetics, Polymorphism, Single-Stranded Conformational, Reverse Transcriptase Polymerase Chain Reaction, Skin Neoplasms/ genetics/metabolism/pathology, Trans-Activators, Tumor Cells, Cultured, beta Catenin|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)|
|Divisions:||Past > Schools > Cell & Molecular Biosciences|
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2002 Lippincott, Williams & Wilkins|
|Deposited On:||14 Sep 2011 09:10|
|Last Modified:||18 Sep 2011 10:22|
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