QUT ePrints

Spatial analysis of biomineralization associated gene expression from the mantle organ of the pearl oyster Pinctada maxima

Gardner, Luke David, Mills, David, Aaron, Wiegand, Leavesley, David, & Abigail, Elizur (2011) Spatial analysis of biomineralization associated gene expression from the mantle organ of the pearl oyster Pinctada maxima. BMC Genomics, 12(455).

View at publisher

Abstract

Background: Biomineralization is a process encompassing all mineral containing tissues produced within an organism. One of the most dynamic examples of this process is the formation of the mollusk shell, comprising a variety of crystal phases and microstructures. The organic component incorporated within the shell is said to dictate this architecture. However general understanding of how this process is achieved remains ambiguous. The mantle is a conserved organ involved in shell formation throughout molluscs. Specifically the mantle is thought to be responsible for secreting the protein component of the shell. This study employs molecular approaches to determine the spatial expression of genes within the mantle tissue to further the elucidation of the shell biomineralization.

Results: A microarray platform was custom generated (PmaxArray 1.0) from the pearl oyster Pinctada maxima. PmaxArray 1.0 consists of 4992 expressed sequence tags (ESTs) originating from mantle tissue. This microarray was used to analyze the spatial expression of ESTs throughout the mantle organ. The mantle was dissected into five discrete regions and analyzed for differential gene expression with PmaxArray 1.0. Over 2000 ESTs were determined to be differentially expressed among the tissue sections, identifying five major expression regions. In situ hybridization validated and further localized the expression for a subset of these ESTs. Comparative sequence similarity analysis of these ESTs revealed a number of the transcripts were novel while others showed significant sequence similarities to previously characterized shell related genes.

Impact and interest:

10 citations in Scopus
Search Google Scholar™
10 citations in Web of Science®

Citation countsare sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

157 since deposited on 12 Sep 2011
62 in the past twelve months

Full-text downloadsdisplays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 45862
Item Type: Journal Article
Additional Information: This work was supported by grants from the Queensland Department of Primary Industries and Fisheries to AE and LG. LG was supported by a QUT Dean’s post graduate award. The authors would also like to thank Bribie Island Aquaculture Research Centre, particularly Hazra Thaggard and Philip Brady for advice and project logistics. Margaret Bernard, from the Queensland Agriculture and Biotechnology Centre, for microarray logistics. Mohammed Amigh, from Queensland Department of Primary Industries, for his assistance with histological preparations. Heidi Mumme for logistics associated with pearl oyster access and maintenance.
Keywords: Biomineralization , mollusk shell, spatial expression
DOI: 10.1186/1471-2164-12-455
ISSN: 1471-2164
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Gene Expression (incl. Microarray and other genome-wide approaches) (060405)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > ZOOLOGY (060800) > Invertebrate Biology (060808)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > FISHERIES SCIENCES (070400) > Aquaculture (070401)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000) > FISHERIES SCIENCES (070400) > Fish Physiology and Genetics (070405)
Divisions: Past > Schools > Cell & Molecular Biosciences
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2011 Gardner et al; licensee BioMed Central Ltd.
Deposited On: 12 Sep 2011 11:11
Last Modified: 06 Sep 2013 16:05

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page