Influence of internal fixator stiffness on murine fracture healing : two types of fracture healing lead to two distinct cellular events and FGF-2 expressions

Ueno, M., Urabe, K., Naruse, K., Uchida, K., Minehara, H., Yamamoto, T., Steck, R., Gregory, L.S., Wullschleger, M.E., Schuetz, M., & Itoman, M. (2011) Influence of internal fixator stiffness on murine fracture healing : two types of fracture healing lead to two distinct cellular events and FGF-2 expressions. Experimental Animals, 60(1), pp. 79-87.

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This study aimed to clarify the relationship between the mechanical environment at the fracture site and endogenous fibroblast growth factor-2 (FGF-2). We compared two types of fracture healing with different callus formations and cellular events using MouseFix(TM) plate fixation systems for murine fracture models. Left femoral fractures were induced in 72 ten-week-old mice and then fixed with a flexible (Group F) or rigid (Group R) Mouse Fix(TM) plate. Mice were sacrificed on days 3, 5, 7, 10, 14, and 21. The callus volumes were measured by 3D micro-CT and tissues were histologically stained with hematoxylin & eosin or safranin-O. Sections from days 3, 5, and 7 were immunostained for FGF-2 and Proliferating Cell Nuclear Antigen (PCNA). The callus in Group F was significantly larger than that in Group R. The rigid plate allowed bone union without a marked external callus or chondrogenesis. The flexible plate formed a large external callus as a result of endochondral ossification. Fibroblastic cells in the granulation tissue on days 5 and 7 in Group F showed marked FGF-2 expression compared with Group R. Fibroblastic cells showed ongoing proliferation in granulation tissue in group F, as indicated by PCNA expression, which explained the relative granulation tissue increase in group F. There were major differences in early phase endogenous FGF-2 expression between these two fracture healing processes, due to different mechanical environments.

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12 citations in Scopus
12 citations in Web of Science®
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ID Code: 46393
Item Type: Journal Article
Refereed: Yes
Additional Information: Ueno, Masaki
Urabe, Ken
Naruse, Kouji
Uchida, Kentaroo
Minehara, Hiroaki
Yamamoto, Takeaki
Steck, Roland
Gregory, Laura
Wullschleger, Martin E
Schuetz, Michael A
Itoman, Moritoshi
Research Support, Non-U.S. Gov't
Experimental animals / Japanese Association for Laboratory Animal Science
Exp Anim. 2011;60(1):79-87.
Additional URLs:
Keywords: Callus formation, fracture, growth factor, internal fixator, mouse
ISSN: 1341-1357
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 11 Oct 2011 22:45
Last Modified: 11 Oct 2011 22:46

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