Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma

Lee, Yen-Ching, Perren, Janeanne, Douglas, Evelyn, Raynor, Michael, Bartley, Maria, Bardy, Peter, & Stephenson, Sally-Anne (2005) Investigation of the expression of the EphB4 receptor tyrosine kinase in prostate carcinoma. BMC Cancer, 5(1).

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The EphB4 receptor tyrosine kinase has been reported as increased in tumours originating from several different tissues and its expression in a prostate cancer xenograft model has been reported. Methods: RT-PCR, western blotting and immunohistochemical techniques were used to examine EphB4 expression and protein levels in human prostate cancer cell lines LNCaP, DU145 and PC3. Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas. Results: All three prostate cancer cell lines expressed the EphB4 gene and protein. EphB4 immunoreactivity in vivo was significantly greater in human prostate cancers as compared with matched normal prostate epithelium and there appeared to be a trend towards increased expression with higher grade disease. Conclusions: EphB4 is expressed in prostate cancer cell lines with increased expression in human prostate cancers when compared with matched normal tissue. EphB4 may therefore be a useful anti-prostate cancer target.

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35 citations in Scopus
28 citations in Web of Science®
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ID Code: 48355
Item Type: Journal Article
Refereed: Yes
Keywords: cancer cell culture, prostate cancer, reverse transcription polymerase chain reaction
DOI: 10.1186/1471-2407-5-119
ISSN: 1471-2407
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Facilities: Banyo Pilot Plant
Copyright Owner: © 2005 Lee et al., licensee BioMed Central Ltd
Copyright Statement: This is an Open Access article distributed under the terms of
the Creative Commons Attribution License (,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Deposited On: 31 Jan 2012 22:23
Last Modified: 19 Dec 2016 02:59

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