High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni
Price, Erin P., Smith, Helen, Huygens, Flavia, & Giffard, Philip M. (2007) High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni. Applied and Environmental Microbiology, 73(10), pp. 3431-3436.
Abstract
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species
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| ID Code: | 48953 |
|---|---|
| Item Type: | Journal Article |
| Keywords: | High Resolution Melt, Genotyping, Campylobacter, CrispR |
| DOI: | 10.1128/AEM.02702-06 |
| ISSN: | 0099-2240 |
| Subjects: | Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) |
| Divisions: | Current > Schools > School of Biomedical Sciences Current > QUT Faculties and Divisions > Faculty of Health Current > Institutes > Institute of Health and Biomedical Innovation |
| Deposited On: | 05 Mar 2012 07:55 |
| Last Modified: | 05 Mar 2012 07:55 |
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