Cyclooxygenase-2 Inhibition Delays the Attainment of Woven Bone Formation Following 4-Point-Bending in the Rat

Gregory, Laura S. & Forwood, Mark R. (2007) Cyclooxygenase-2 Inhibition Delays the Attainment of Woven Bone Formation Following 4-Point-Bending in the Rat. Calcified Tissue International, 80(3), pp. 176-183.


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Fracture healing is retarded in the presence of cyclooxygenase-2 (COX-2) inhibitors, demonstrating an important role of COX-2 in trauma-induced woven bone adaptation. The aim of this experiment was to determine the influence of COX-2 inhibition on the remodeling and consolidation of non-traumatic woven bone produced by mechanical loading. A periosteal woven bone callus was initiated in the right tibia of female Wistar rats following a single bout of four-point-bending, applied as a haversine wave for 300 cycles at a frequency of 2Hz and a magnitude of 65N. Daily injections of Vehicle (VEH: polyethyleneglycol) or the COX-2 inhibitor, DFU (2.0 and i.p.), commenced 7 days postloading, and tibiae were examined 2, 3, 4 and 5 weeks postloading. Tibiae were dissected, embedded in polymethylmethacrylate and sectioned for histomorphometric analysis of periosteal woven bone. No significant difference in peak woven bone area was observed between DFU-treated and VEH rats. But treatment with DFU resulted in a temporal defect in woven bone formation, where the achievement of peak woven bone area was delayed by one week. Woven bone remodeling was observed in DFU-treated rats at 21 days post-loading, demonstrating that remodeling of the periosteal callus is not prevented in the presence of a COX-2 inhibitor in the rat. We conclude that COX-2 inhibition does not significantly disrupt the mechanism of woven bone remodeling, but alters its timing.

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13 citations in Scopus
10 citations in Web of Science®
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ID Code: 4914
Item Type: Journal Article
Refereed: Yes
Keywords: woven bone, cyclooxygenase isoforms, bone remodeling, periosteal callus, 4, point, bending
DOI: 10.1007/s00223-006-0170-8
ISSN: 1432-0827
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > HUMAN MOVEMENT AND SPORTS SCIENCE (110600) > Biomechanics (110601)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2007 Springer
Copyright Statement: The original publication is available at SpringerLink
Deposited On: 04 Sep 2006 00:00
Last Modified: 29 Feb 2012 14:12

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