Expression pattern of the Alpha-Kafirin promoter coupled with a signal peptide from Sorghum bicolor L. Moench
Ahmad, Norazlina, Sant, Rajnesh, Bokan, Milovan, Steadman, Kathryn J., & Godwin, Ian D. (2012) Expression pattern of the Alpha-Kafirin promoter coupled with a signal peptide from Sorghum bicolor L. Moench. Journal of Biomedicine and Biotechnology, 2012, Article ID 752391.
Regulatory sequences with endosperm specificity are essential for foreign gene expression in the desired tissue for both grain quality improvement and molecular pharming. In this study, promoters of seed storage α-kafirin genes coupled with signal sequence (ss) were isolated from Sorghum bicolor L. Moench genomic DNA by PCR. The α-kafirin promoter (α-kaf) contains endosperm specificity-determining motifs, prolamin-box, the O2-box 1, CATC, and TATA boxes required for α-kafirin gene expression in sorghum seeds. The constructs pMB-Ubi-gfp and pMB-kaf-gfp were microprojectile bombarded into various sorghum and sweet corn explants. GFP expression was detected on all explants using the Ubi promoter but only in seeds for the α-kaf promoter. This shows that the α-kaf promoter isolated was functional and demonstrated seed-specific GFP expression. The constructs pMB-Ubi-ss-gfp and pMB-kaf-ss-gfp were also bombarded into the same explants. Detection of GFP expression showed that the signal peptide (SP)::GFP fusion can assemble and fold properly, preserving the fluorescent properties of GFP.
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|Item Type:||Journal Article|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities|
|Copyright Owner:||Copyright © 2012 Norazlina Ahmad et al.|
|Copyright Statement:||This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Deposited On:||12 Apr 2012 00:29|
|Last Modified:||02 Dec 2016 04:58|
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