Effects of oxygen on zonal marker expression in human articular chondrocytes
Schrobback, K., Malda, J. , Crawford, R. W., Upton, Z., Leavesley, D. I., & Klein, T. J. (2012) Effects of oxygen on zonal marker expression in human articular chondrocytes. Tissue Engineering Part A, 18(9-10), pp. 920-933.
Articular cartilage is organized in depth zones with phenotypically distinct subpopulations of chondrocytes that are exposed to different oxygen tensions. Despite growing evidence of the critical role for oxygen in chondrogenesis, little is known about its effect on chondrocytes from different zones. This study evaluates zonal marker expression of human articular chondrocytes from different zones under various oxygen tensions. Chondrocytes isolated from full-thickness, superficial, and middle/deep cartilage from knee replacement surgeries were expanded and redifferentiated under hypoxic (5% O 2) or normoxic (20% O 2) conditions. Differentiation under hypoxia increased expression of hypoxia-inducible factors 1alpha and 2alpha and accumulation of extracellular matrix, particularly in middle/deep chondrocytes, and favored re-expression of proteoglycan 4 by superficial chondrocytes compared with middle/deep cells. Zone-dependent expression of clusterin varied with culture duration. These results demonstrate that zonal chondrocytes retain important phenotypic differences during in vitro cultivation, and that these characteristics can be improved by altering the oxygen environment. However, transcript levels for pleiotrophin, cartilage intermediate layer protein, and collagen type X were similar between zones, challenging their reliability as zonal markers for tissue-engineered cartilage from osteoarthritis patients. Key factors including oxygen tension and cell source should be considered to prescribe zone-specific properties to tissue-engineered cartilage. © 2012, Mary Ann Liebert, Inc.
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|Item Type:||Journal Article|
|Subjects:||Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > MECHANICAL ENGINEERING (091300)|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering|
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2012 Mary Ann Liebert, Inc. Publishers|
|Copyright Statement:||This is a copy of an article published in the Tissue Engineering Part A (C) 2012 (copyright Mary Ann Liebert, Inc.); Tissue Engineering Part A is available online at: http://www.liebertonline.com.|
|Deposited On:||17 May 2012 12:06|
|Last Modified:||01 Jun 2012 17:03|
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