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Phosphorylation of ATPase subunits of the 26S proteasome

Mason, Grant, Murray, Rachael, Pappin, Daryl, & Rivett, A. Jennifer (1998) Phosphorylation of ATPase subunits of the 26S proteasome. FEBS Letters, 430(3), pp. 269-274.

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Abstract

Abstract The 26S proteasome complex plays a major role in the non-lysosomal degradation of intracellular proteins. Purified 26S proteasomes give a pattern of more than 40 spots on 2DPAGE gels. The positions of subunits have been identified by mass spectrometry of tryptic peptides and by immunoblotting with subunit-specific antipeptide antibodies. Two-dimensional polyacrylamide gel electrophoresis of proteasomes immunoprecipitated from [32P]phosphate-labelled human embryo lung L-132 cells revealed the presence of at least three major phosphorylated polypeptides among the regulatory subunits as well as the C8 and C9 components of the core 20S proteasome. Comparison with the positions of the regulatory polypeptides revealed a minor phosphorylated form to be S7 (MSS1). Antibodies against S4, S6 (TBP7) and S12 (MOV34) all cross-reacted at the position of major phosphorylated polypeptides suggesting that several of the ATPase subunits may be phosphorylated. The phosphorylation of S4 was confirmed by double immunoprecipitation experiments in which 26S oteasomes were immunoprecipitated as above and dissociated and Antibodies against the non-ATPase subunit S10, which has been suggested by others to be phosphorylated, did not coincide with the position of a phosphorylated polypeptide. Some differences were observed in the 2D-PAGE pattern of proteasomes immunoprecipitated from cultured cells compared to purified rat liver 26S proteasomes suggesting possible differences in subunit compositions of 26S proteasomes.

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ID Code: 50495
Item Type: Journal Article
Keywords: Proteasome, Phosphorylation, Mass spectrometry, ATPase
DOI: 10.1016/S0014-5793(98)00676-0
ISSN: 0014-5793
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Enzymes (060107)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 22 May 2012 23:48
Last Modified: 22 May 2012 23:48

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