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A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex

Vaquette, Cedryck, Fan, Wei, Xiao, Yin, Hamlet, Stephen , Hutmacher, Dietmar W., & Ivanovski, Saso (2012) A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex. Biomaterials, 33(22), pp. 5560-5573.

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Abstract

This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. These scaffolds also demonstrated better attachment onto the dentin surface compared to no attachment when no cell sheets were used. In addition, immunohistochemistry revealed the presence of CEMP1 protein at the interface with the dentine. These results demonstrated that the combination of multiple PDL cell sheets and a biphasic scaffold allows the simultaneous delivery of the cells necessary for in vivo regeneration of alveolar bone, periodontal ligament and cementum. © 2012 Elsevier Ltd.

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15 citations in Scopus
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15 citations in Web of Science®

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ID Code: 50922
Item Type: Journal Article
Keywords: Biphasic scaffold, Cell sheet, Cementogenesis, Electrospinning, Periodontal regeneration, Alveolar bones, Athymic, Biphasic scaffolds, Cell sheet harvesting, Cell-based, Electrospun membranes, Fused deposition modeling, Immunohistochemistry, In-vitro, In-vivo, Osteoblast cultures, Periodontal ligament, Bone, Cell culture, Cell death, Osteoblasts, Tissue, Scaffolds (biology), alveolar bone, animal cell, animal experiment, article, bone mineralization, bone regeneration, cementum, computer assisted tomography, controlled study, dentin, histology, in vitro study, male, nonhuman, osteoblast, priority journal, rat, Rattus
DOI: 10.1016/j.biomaterials.2012.04.038
ISSN: 0142-9612
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 18 Jun 2012 08:11
Last Modified: 10 Oct 2012 11:50

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