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Engineering of vascularized adipose constructs

Wiggenhauser, Paul Severin, Müller, Daniel F, Melchels, Ferry P.W., Egaña, José T, Storck, Katharina, Mayer, Helena, Leuthner, Peter, Skodacek, Daniel, Hopfner, Ursula, Machens, Hans G, Staudenmaier, Rainer, & Schantz, Jan Thorsten (2012) Engineering of vascularized adipose constructs. Cell and Tissue Research, 347(3), pp. 747-757.

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Abstract

Adipose tissue engineering offers a promising alternative to the current surgical techniques for the treatment of soft tissue defects. It is a challenge to find the appropriate scaffold that not only represents a suitable environment for cells but also allows fabrication of customized tissue constructs, particularly in breast surgery. We investigated two different scaffolds for their potential use in adipose tissue regeneration. Sponge-like polyurethane scaffolds were prepared by mold casting with methylal as foaming agent, whereas polycaprolactone scaffolds with highly regular stacked-fiber architecture were fabricated with fused deposition modeling. Both scaffold types were seeded with human adipose tissuederived precursor cells, cultured and implanted in nude mice using a femoral arteriovenous flow-through vessel loop for angiogenesis. In vitro, cells attached to both scaffolds and differentiated into adipocytes. In vivo, angiogenesis and adipose tissue formation were observed throughout both constructs after 2 and 4 weeks, with angiogenesis being comparable in seeded and unseeded constructs. Fibrous tissue formation and adipogenesis were more pronounced on polyurethane foam scaffolds than on polycaprolactone prototyped scaffolds. In conclusion, both scaffold designs can be effectively used for adipose tissue engineering.

Impact and interest:

5 citations in Scopus
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4 citations in Web of Science®

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146 since deposited on 28 Jun 2012
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ID Code: 51169
Item Type: Journal Article
Keywords: Adipose tissue engineering, Polycaprolactone , Polyurethane , Vessel loop, Angiogenesis
DOI: 10.1007/s00441-011-1226-2
ISSN: 0302-766X
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2012 Springer-Verlag.
Deposited On: 28 Jun 2012 17:12
Last Modified: 28 Jun 2012 21:59

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