Fast fourier analysis of structural organization in decellularized cartilage-on-bone laminates
Heidarkhan Tehrani, Ashkan, Singh, Sanjleena, Xiao, Yin, & Oloyede, Adekunle (2012) Fast fourier analysis of structural organization in decellularized cartilage-on-bone laminates. In Merrell , R, Shin, D. G, & Hamza , M. H (Eds.) Proceedings of the IASTED International Symposia Imaging and Signal Processing in Health Care and Technology (ISPHT 2012), IEEE Engineering in Medicine and Biology Society , Baltimore, USA , pp. 71-77.
Denaturation of tissues can provide a unique biological environment for regenerative medicine application only if minimal disruption of their microarchitecture is achieved during the decellularization process. The goal is to keep the structural integrity of such a construct as functional as the tissues from which they were derived. In this work, cartilage-on-bone laminates were decellularized through enzymatic, non-ionic and ionic protocols. This work investigated the effects of decellularization process on the microarchitecture of cartiligous extracellular matrix; determining the extent of how each process deteriorated the structural organization of the network. High resolution microscopy was used to capture cross-sectional images of samples prior to and after treatment. The variation of the microarchitecture was then analysed using a well defined fast Fourier image processing algorithm. Statistical analysis of the results revealed how significant the alternations among aforementioned protocols were (p < 0.05). Ranking the treatments by their effectiveness in disrupting the ECM integrity, they were ordered as: Trypsin> SDS> Triton X-100.
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|Item Type:||Conference Paper|
|Keywords:||Decellularization, Extracellular matrix, Image processing, Fast Fourier transform, Cartilage-on-bone laminate|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Deposited On:||01 Jul 2012 23:49|
|Last Modified:||12 Jun 2013 14:53|
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