Investigating the potential value of individual parameters of histological grading systems in a sheep model of cartilage damage : the modified Mankin method

Moody, Hayley R., Heard, Cyril J., Frank, Cyril B., Shrive, Nigel G., & Oloyede, Adekunle (2012) Investigating the potential value of individual parameters of histological grading systems in a sheep model of cartilage damage : the modified Mankin method. Journal of Anatomy, 221(1), pp. 47-54.

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A total histological grade does not necessarily distinguish between different manifestations of cartilage damage or degeneration. An accurate and reliable histological assessment method is required to separate normal and pathological tissue within a joint during treatment of degenerative joint conditions and to sub-classify the latter in meaningful ways. The Modified Mankin method may be adaptable for this purpose. We investigated how much detail may be lost by assigning one composite score/grade to represent different degenerative components of the osteoarthritic condition. We used four ovine injury models (sham surgery, anterior cruciate ligament/medial collateral ligament instability, simulated anatomic anterior cruciate ligament reconstruction and meniscal removal) to induce different degrees and potentially 'types' (mechanisms) of osteoarthritis. Articular cartilage was systematically harvested, prepared for histological examination and graded in a blinded fashion using a Modified Mankin grading method. Results showed that the possible permutations of cartilage damage were significant and far more varied than the current intended use that histological grading systems allow. Of 1352 cartilage specimens graded, 234 different manifestations of potential histological damage were observed across 23 potential individual grades of the Modified Mankin grading method. The results presented here show that current composite histological grading may contain additional information that could potentially discern different stages or mechanisms of cartilage damage and degeneration in a sheep model. This approach may be applicable to other grading systems.

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10 citations in Scopus
11 citations in Web of Science®
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ID Code: 51409
Item Type: Journal Article
Refereed: Yes
Keywords: Cartilage, Grading, Histology, Modified Mankin, Osteoarthritis, Ovine
DOI: 10.1111/j.1469-7580.2012.01513.x
ISSN: 00218782 (ISSN)
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: © 2012 The Authors. Journal of Anatomy © 2012 Anatomical Society.
Deposited On: 03 Jul 2012 04:40
Last Modified: 12 Jun 2013 14:54

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