ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells
Leong, David Tai , Abraham, Mohan Chothirakottu , Gupta, Anurag , Lim, Thiam-Chye , Chew, Fook Tim , & Hutmacher, Dietmar (2012) ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells. Journal of Cellular Biochemistry, 113(8), pp. 2744-2753.
The regulatory pathways involved in maintaining the pluripotency of embryonic stem cells are partially known, whereas the regulatory pathways governing adult stem cells and their "stem-ness" are characterized to an even lesser extent. We, therefore, screened the transcriptome profiles of 20 osteogenically induced adult human adipose-derived stem cell (ADSC) populations and investigated for putative transcription factors that could regulate the osteogenic differentiation of these ADSC. We studied a subgroup of donors' samples that had a disparate osteogenic response transcriptome from that of induced human fetal osteoblasts and the rest of the induced human ADSC samples. From our statistical analysis, we found activating transcription factor 5 (ATF5) to be significantly and consistently down-regulated in a randomized time-course study of osteogenically differentiated adipose-derived stem cells from human donor samples. Knockdown of ATF5 with siRNA showed an increased sensitivity to osteogenic induction. This evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose-derived stem cells. To our knowledge, this is the first report that indicates a novel role of transcription factors in regulating osteogenic differentiation in adult or tissue specific stem cells. © 2012 Wiley Periodicals, Inc.
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|Item Type:||Journal Article|
|Keywords:||Adipose derived stem cells, ATF5, Osteogenic differentiation|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)|
|Divisions:||Current > Schools > School of Chemistry, Physics & Mechanical Engineering|
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Deposited On:||05 Jul 2012 08:09|
|Last Modified:||13 Jun 2013 00:54|
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