A mass spectrometric assay for the quantification of neuropeptide PYY in plasma
Xi, Li, Jin, Yaping, Josh, Peter, Parker, Edward A., Herzog, Herbert, & Colgrave, Michelle (2012) A mass spectrometric assay for the quantification of neuropeptide PYY in plasma. Analytical Methods, 4(3), pp. 714-720.
A multiple reaction monitoring mass spectrometric assay for the quantification of PYY in human plasma has been developed. A two stage sample preparation protocol was employed in which plasma containing the full length neuropeptide was first digested using trypsin, followed by solid-phase extraction to extract the digested peptide from the complex plasma matrix. The peptide extracts were analysed by LC-MS using multiple reaction monitoring to detect and quantify PYY. The method has been validated for plasma samples, yielding linear responses over the range 5–1,000 ng mL−1. The method is rapid, robust and specific for plasma PYY detection.
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|Item Type:||Journal Article|
|Keywords:||spectrometric assay, PYY, plasma|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000)|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Deposited On:||05 Jul 2012 22:10|
|Last Modified:||12 Jun 2013 14:54|
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