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A tissue engineering solution for segmental defect regeneration in load-bearing long bones

Reichert, J. C., Cipitria, A., Epari, D. R., Saifzadeh, S., Krishnakanth, P., Berner, A., Woodruff, Maria A., Schell, H., Mehta, M., Schuetz, Michael, Duda, G. N., & Hutmacher, D. W. (2012) A tissue engineering solution for segmental defect regeneration in load-bearing long bones. Science Translational Medicine, 4(141), 141ra93.

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Abstract

The reconstruction of large defects (>10 mm) in humans usually relies on bone graft transplantation. Limiting factors include availability of graft material, comorbidity, and insufficient integration into the damaged bone. We compare the gold standard autograft with biodegradable composite scaffolds consisting of medical-grade polycaprolactone and tricalcium phosphate combined with autologous bone marrow-derived mesenchymal stem cells (MSCs) or recombinant human bone morphogenetic protein 7 (rhBMP-7). Critical-sized defects in sheep - a model closely resembling human bone formation and structure - were treated with autograft, rhBMP-7, or MSCs. Bridging was observed within 3 months for both the autograft and the rhBMP-7 treatment. After 12 months, biomechanical analysis and microcomputed tomography imaging showed significantly greater bone formation and superior strength for the biomaterial scaffolds loaded with rhBMP-7 compared to the autograft. Axial bone distribution was greater at the interfaces. With rhBMP-7, at 3 months, the radial bone distribution within the scaffolds was homogeneous. At 12 months, however, significantly more bone was found in the scaffold architecture, indicating bone remodeling. Scaffolds alone or with MSC inclusion did not induce levels of bone formation comparable to those of the autograft and rhBMP-7 groups. Applied clinically, this approach using rhBMP-7 could overcome autograft-associated limitations.

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34 citations in Scopus
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15 citations in Web of Science®

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ID Code: 52766
Item Type: Journal Article
DOI: 10.1126/scitranslmed.3003720
ISSN: 19466234 (ISSN)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2012 American Association for the Advancement of Science
Deposited On: 27 Jul 2012 14:37
Last Modified: 13 Jun 2013 01:04

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