Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes
Mlalazi, Bulukani, Welsch, Ralf, Namanya, Priver, Khanna, Harjeet K., Geijskes, R. Jason, Harrison, Mark D., Harding, Robert M., Dale, James L., & Bateson, Marion (2012) Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes. Planta.
Carotenoids occur in all photosynthetic organisms where they protect photosystems from auto-oxidation, participate in photosynthetic energy-transfer and are secondary metabolites. Of the more than 600 known plant carotenoids, few can be converted into vitamin A by humans and so these pro-vitamin A carotenoids (pVAC) are important in human nutrition. Phytoene synthase (PSY) is a key enzyme in the biosynthetic pathway of pVACs and plays a central role in regulating pVAC accumulation in the edible portion of crop plants. Bananas are a major commercial crop and serve as a staple crop for more than 30 million people. There is natural variation in fruit pVAC content across different banana cultivars, but this is not well understood. Therefore, we isolated PSY genes from banana cultivars with relatively high (cv. Asupina) and low (cv. Cavendish) pVAC content. We provide evidence that PSY in banana is encoded by two paralogs (PSY1 and PSY2), each with a similar gene structure to homologous genes in other monocots. Further, we demonstrate that PSY2 is more highly expressed in fruit pulp compared to leaf. Functional analysis of PSY1 and PSY2 in rice callus and E. coli demonstrate that both genes encode functional enzymes, and that Asupina PSYs have approximately twice the enzymatic activity of the corresponding Cavendish PSYs. These results suggest that differences in PSY enzyme activity contribute significantly to the differences in Asupina and Cavendish fruit pVAC content. Importantly, Asupina PSY genes could potentially be used to generate new cisgenic or intragenic banana cultivars with enhanced pVAC content.
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|Item Type:||Journal Article|
|Keywords:||Biofortification, Carotene, Cisgenic, Intragenic, Isoprenoid, Metabolic engineering|
|Subjects:||Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > AGRICULTURAL AND VETERINARY SCIENCES (070000)
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2012 Springer-Verlag|
|Copyright Statement:||The original publication is available at SpringerLink
|Deposited On:||05 Aug 2012 23:29|
|Last Modified:||10 Aug 2012 08:43|
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