Identification of vitronectin as a novel insulin-like growth factor-II binding protein

Upton, Zee, Webb, Helen, Hale, Kathryn , Yandell, Catherine, McMurtry, John, Francis , Geoffrey, & Ballard, F. John (1999) Identification of vitronectin as a novel insulin-like growth factor-II binding protein. Endocrinology, 140(6), pp. 2928-2931.

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We have previously reported the presence of a 70 kDa insulin-like growth factor (IGF)-II-specific binding protein in chicken serum using Western ligand blotting approaches. In order to ascertain the identity of this 70 kDa IGF-II binding species, the protein has been purified from chicken serum using a combination of ion-exchange and gel-permeation chromatography. Interestingly, amino acid sequencing of the purified protein revealed that it has the same N-terminal sequence as chicken vitronectin (VN). The protein has the ability to specifically bind IGF-II and not IGF-I as determined by ligand blotting, cross-linking and competitive binding assay approaches. In addition, the protein binds 125I-des(l-6)-IGF-II, suggesting that the interaction with IGF-II is different to those with other characterized IGF-binding proteins. Importantly, we have ascertained that both human and bovine VN also specifically bind IGF-II. These results are particularly relevant in the light of the recent report that the urokinase-type plasminogen activator receptor, a protein that also binds VN, has been shown to associate with the cation-independent mannose-6-phosphate/GF-II receptor and suggest a possible role for IGF-II in cell adhesion and invasion.

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37 citations in Web of Science®
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ID Code: 53056
Item Type: Journal Article
Refereed: Yes
Keywords: somatomedin binding protein 2, vitronectin, amino acid sequence, cross linking, gel permeation chromatography, ion exchange chromatography, protein binding, protein purification, receptor binding, Western blotting, Insulin-Like Growth Factor Binding Proteins, Insulin-Like Growth Factor II, Molecular Sequence Data
DOI: 10.1210/en.140.6.2928
ISSN: 0013-7227
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1999 The Endocrine Society
Deposited On: 12 Aug 2012 23:28
Last Modified: 12 Aug 2012 23:28

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