A portable approach for the surveillance of dengue virus-infected mosquitoes

Muller, D.A., Frentiu, F.D., Rojas, A., Moreira, L.A., O'Neill, S.L., & Young , P.R. (2012) A portable approach for the surveillance of dengue virus-infected mosquitoes. Journal of Virological Methods, 183(1), pp. 90-93.

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Dengue virus is the most significant human viral pathogen spread by the bite of an infected mosquito. With no vaccine or antiviral therapy currently available, disease prevention relies largely on surveillance and mosquito control. Preventing the onset of dengue outbreaks and effective vector management would be considerably enhanced through surveillance of dengue virus prevalence in natural mosquito populations. However, current approaches to the identification of virus in field-caught mosquitoes require relatively slow and labor intensive techniques such as virus isolation or RT-PCR involving specialized facilities and personnel. A rapid and portable method for detecting dengue virus-infected mosquitoes is described. Using a hand held battery operated homogenizer and a dengue diagnostic rapid strip the viral protein NS1 was detected as a marker of dengue virus infection. This method could be performed in less than 30 min in the field, requiring no downstream processing, and is able to detect a single infected mosquito in a pool of at least 50 uninfected mosquitoes. The method described in this study allows rapid, real-time monitoring of dengue virus presence in mosquito populations and could be a useful addition to effective monitoring and vector control responses.

Impact and interest:

11 citations in Scopus
10 citations in Web of Science®
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ID Code: 54043
Item Type: Journal Article
Refereed: Yes
Keywords: dengue virus, mosquito, vector control
DOI: 10.1016/j.jviromet.2012.03.033
ISSN: 0166-0934
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 11 Oct 2012 23:57
Last Modified: 14 Oct 2012 22:38

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