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Fabrication of a corneal-limbal tissue substitute using silk fibroin

Bray, Laura J., George, Karina A., Suzuki, Shuko, Chirila, Traian V., & Harkin, Damien G. (2013) Fabrication of a corneal-limbal tissue substitute using silk fibroin. In Wright, Bernice & Connon, Che (Eds.) Methods in Molecular Biology [Corneal Regenerative Medicine: Methods and Protocols]. Human Press Ltd..

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Abstract

Fibroin extracted from silkworm cocoon silk provides an intriguing and potentially important biomaterial for corneal reconstruction. In the present chapter we outline our methods for producing a composite of two fibroin-based materials that supports the co-cultivation of human limbal epithelial (HLE) cells and human limbal stromal (HLS) cells. The resulting tissue substitute consists of a stratified epithelium overlying a three-dimensional arrangement of extracellular matrix components (principally ‘degummed’ fibroin fibers) and mesenchymal stromal cells. This tissue substitute is currently being evaluated as a tool for reconstructing the corneal limbus and corneal epithelium.

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ID Code: 54162
Item Type: Book Chapter
Keywords: Silk fibroin, Corneal limbus, Corneal epithelium, Limbal epithelium, Mesenchymal stromal cells
DOI: 10.1007/978-1-62703-432-6_11
ISSN: 1064-3745
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400) > Regenerative Medicine (incl. Stem Cells and Tissue Engineering) (100404)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300) > Ophthalmology (111301)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Funding:
Copyright Owner: Copyright 2012 Please consult the authors.
Deposited On: 15 Oct 2012 07:44
Last Modified: 16 Oct 2014 19:26

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