QUT ePrints

Fabrication of a corneal-limbal tissue substitute using silk fibroin

Bray, Laura J., George, Karina A., Suzuki, Shuko, Chirila, Traian V., & Harkin, Damien G. (2013) Fabrication of a corneal-limbal tissue substitute using silk fibroin. In Wright, Bernice & Connon, Che (Eds.) Methods in Molecular Biology [Corneal Regenerative Medicine: Methods and Protocols]. Human Press Ltd..

[img] Accepted Version (PDF 5MB)
Administrators only | Request a copy from author

    View at publisher

    Abstract

    Fibroin extracted from silkworm cocoon silk provides an intriguing and potentially important biomaterial for corneal reconstruction. In the present chapter we outline our methods for producing a composite of two fibroin-based materials that supports the co-cultivation of human limbal epithelial (HLE) cells and human limbal stromal (HLS) cells. The resulting tissue substitute consists of a stratified epithelium overlying a three-dimensional arrangement of extracellular matrix components (principally ‘degummed’ fibroin fibers) and mesenchymal stromal cells. This tissue substitute is currently being evaluated as a tool for reconstructing the corneal limbus and corneal epithelium.

    Impact and interest:

    1 citations in Scopus
    Search Google Scholar™

    Citation countsare sourced monthly from Scopus and Web of Science® citation databases.

    These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

    Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

    ID Code: 54162
    Item Type: Book Chapter
    Keywords: Silk fibroin, Corneal limbus, Corneal epithelium, Limbal epithelium, Mesenchymal stromal cells
    DOI: 10.1007/978-1-62703-432-6_11
    ISSN: 1064-3745
    Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
    Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > MEDICAL BIOTECHNOLOGY (100400) > Regenerative Medicine (incl. Stem Cells and Tissue Engineering) (100404)
    Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300) > Ophthalmology (111301)
    Divisions: Current > Schools > School of Biomedical Sciences
    Current > QUT Faculties and Divisions > Faculty of Health
    Current > Institutes > Institute of Health and Biomedical Innovation
    Funding:
    Copyright Owner: Copyright 2012 Please consult the authors.
    Deposited On: 15 Oct 2012 17:44
    Last Modified: 09 Apr 2014 22:21

    Export: EndNote | Dublin Core | BibTeX

    Repository Staff Only: item control page