Fabrication of a corneal-limbal tissue substitute using silk fibroin
Bray, Laura J., George, Karina A., Suzuki, Shuko, Chirila, Traian V., & Harkin, Damien G. (2013) Fabrication of a corneal-limbal tissue substitute using silk fibroin. In Wright, Bernice & Connon, Che (Eds.) Methods in Molecular Biology [Corneal Regenerative Medicine: Methods and Protocols]. Human Press Ltd..
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Fibroin extracted from silkworm cocoon silk provides an intriguing and potentially important biomaterial for corneal reconstruction. In the present chapter we outline our methods for producing a composite of two fibroin-based materials that supports the co-cultivation of human limbal epithelial (HLE) cells and human limbal stromal (HLS) cells. The resulting tissue substitute consists of a stratified epithelium overlying a three-dimensional arrangement of extracellular matrix components (principally ‘degummed’ fibroin fibers) and mesenchymal stromal cells. This tissue substitute is currently being evaluated as a tool for reconstructing the corneal limbus and corneal epithelium.
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