Antioxidant supplementation reduces skeletal muscle mitochondrial biogenesis

Strobel, Natalie, Peake, Jonathan, Matsumoto, Aya, Marsh, Susan, Coombes, Jeff S., & Wadley, Glenn (2011) Antioxidant supplementation reduces skeletal muscle mitochondrial biogenesis. Medicine and Science in Sports and Exercise, 43(6), pp. 1017-1024.

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Purpose: Exercise increases the production of reactive oxygen species (ROS) in skeletal muscle, and athletes often consume antioxidant supplements in the belief they will attenuate ROS-related muscle damage and fatigue during exercise. However, exercise-induced ROS may regulate beneficial skeletal muscle adaptations, such as increased mitochondrial biogenesis. We therefore investigated the effects of long-term antioxidant supplementation with vitamin E and alpha-lipoic acid on changes in markers of mitochondrial biogenesis in the skeletal muscle of exercise-trained and sedentary rats. Methods: Male Wistar rats were divided into four groups: 1) sedentary control diet, 2) sedentary antioxidant diet, 3) exercise control diet, and 4) exercise antioxidant diet. Animals ran on a treadmill 4 d.wk(-1) at similar to 70% V (over dot)O(2max) for up to 90 min.d(-1) for 14 wk. Results: Consistent with the augmentation of skeletal muscle mitochondrial biogenesis and antioxidant defenses, after training there were significant increases in peroxisome proliferator-activated receptor F coactivator 1 alpha (PGC-1 alpha) messenger RNA (mRNA) and protein, cytochrome C oxidase subunit IV (COX IV) and cytochrome C protein abundance, citrate synthase activity, Nfe2l2, and SOD2 protein (P < 0.05). Antioxidant supplementation reduced PGC-1 alpha mRNA, PGC-1 alpha and COX IV protein, and citrate synthase enzyme activity (P < 0.05) in both sedentary and exercise-trained rats. Conclusions: Vitamin E and alpha-lipoic acid supplementation suppresses skeletal muscle mitochondrial biogenesis, regardless of training status.

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ID Code: 54289
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: exercise, oxidative stress, antioxidants, mitochondrial biogenesis
DOI: 10.1249/MSS.0b013e318203afa3
ISSN: 1530-0315 (online) 0195-9131 (print)
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > HUMAN MOVEMENT AND SPORTS SCIENCE (110600) > Exercise Physiology (110602)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Coyright 2011 The American College of Sports Medicine
Deposited On: 24 Oct 2012 23:19
Last Modified: 26 Oct 2012 00:12

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