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Real-time measurement of F-Actin remodelling during exocytosis using lifeact-EGFP transgenic animals

Jang, Yujin, Soekmadji, Carolina, Mitchell, Justin M., Thomas, Walter G., & Thorn, Peter (2012) Real-time measurement of F-Actin remodelling during exocytosis using lifeact-EGFP transgenic animals. PLOS One, 7(7), e39815.

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Abstract

F-actin remodelling is essential for a wide variety of cell processes. It is important in exocytosis, where F-actin coats fusing exocytic granules. The purpose of these F-actin coats is unknown. They may be important in stabilizing the fused granules, they may play a contractile role and promote expulsion of granule content and finally may be important in endocytosis. To elucidate these functions of F-actin remodelling requires a reliable method to visualize F-actin dynamics in living cells. The recent development of Lifeact-EGFP transgenic animals offers such an opportunity. Here, we studied the characteristics of exocytosis in pancreatic acinar cells obtained from the Lifeact-EGFP transgenic mice. We show that the time-course of agonist-evoked exocytic events and the kinetics of each single exocytic event are the same for wild type and Lifeact-EGFP transgenic animals. We conclude that Lifeact-EGFP animals are a good model to study of exocytosis and reveal that F-actin coating is dependent on the de novo synthesis of F-actin and that development of actin polymerization occurs simultaneously in all regions of the granule. Our insights using the Lifeact-EGFP mice demonstrate that F-actin coating occurs after granule fusion and is a granule-wide event.

Impact and interest:

3 citations in Scopus
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2 citations in Web of Science®

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36 since deposited on 01 Nov 2012
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ID Code: 54483
Item Type: Journal Article
Keywords: Ca2+, exocytosis, actin
DOI: 10.1371/journal.pone.0039815
ISSN: 1932-6203
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2012 The Authors
Deposited On: 02 Nov 2012 08:43
Last Modified: 21 Mar 2013 07:13

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