Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant

Owor, B. E., Martin, D. P., Shepherd, D. N., Edema, R., Monjane, A. L., Rybicki, E. P., Thomson, J. A., & Varsani, A. (2007) Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. Journal of General Virology, 88(11), pp. 3154-3165.

View at publisher


Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants - MSV subtypes A1 -A6 - causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR-restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1 UgIII, accounted for >60% of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species. © 2007 SGM.

Impact and interest:

45 citations in Scopus
Search Google Scholar™
45 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 54932
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996): 35
Export Date: 12 November 2012
Source: Scopus
DOI: 10.1099/vir.0.83144-0
Deposited On: 20 Nov 2012 02:46
Last Modified: 29 May 2013 16:53

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page